干扰物对猫、牛、狗和马血清肌酐浓度测定中动力学Jaffé反应及酶比色法的影响。
Effects of interferents on the kinetic Jaffé reaction and an enzymatic colorimetric test for serum creatinine concentration determination in cats, cows, dogs and horses.
作者信息
Jacobs R M, Lumsden J H, Taylor J A, Grift E
机构信息
Department of Pathology, Ontario Veterinary College, University of Guelph.
出版信息
Can J Vet Res. 1991 Apr;55(2):150-4.
The effects of acetoacetic acid, acetone, bilirubin, beta-carotene, three cephalospoprin antibiotics, glucose, hemoglobin and lipid on the kinetic Jaffé reaction and an enzymatic reaction for the determination of creatinine concentration were studied in bovine, canine, feline and equine serum. There were no obvious species' differences. The kinetic Jaffé reaction was unaffected by the addition of beta-carotene and hemoglobin. Acetone, cefazolin, cefoxitin, ceftiofur and glucose caused a positive bias while acetoacetic acid, bilirubin, and lipid caused a negative bias when added to the kinetic Jaffé reaction. The enzymatic reaction was unaffected by the addition of acetoacetic acid, acetone, beta-carotene, cefazolin, cefoxitin, glucose and hemoglobin while added lipid, bilirubin and ceftiofur caused a negative bias in the test results. Over all species and interferents, there was no difference in the precision of the two assay methods. In a series of sera from hospitalized patients, the two methods were highly correlated in a linear fashion. The enzymatic creatinine assay deals effectively with most interferents but has a greater cost and shorter shelf-life compared with the kinetic Jaffé reaction.
在牛、犬、猫和马的血清中，研究了乙酰乙酸、丙酮、胆红素、β-胡萝卜素、三种头孢菌素抗生素、葡萄糖、血红蛋白和脂质对用于测定肌酐浓度的动力学Jaffe反应和酶促反应的影响。没有明显的物种差异。添加β-胡萝卜素和血红蛋白对动力学Jaffe反应没有影响。添加丙酮、头孢唑林、头孢西丁、头孢噻呋和葡萄糖会导致正偏差，而添加乙酰乙酸、胆红素和脂质会导致动力学Jaffe反应出现负偏差。添加乙酰乙酸、丙酮、β-胡萝卜素、头孢唑林、头孢西丁、葡萄糖和血红蛋白对酶促反应没有影响，而添加脂质、胆红素和头孢噻呋会导致检测结果出现负偏差。在所有物种和干扰物中，两种检测方法的精密度没有差异。在一系列住院患者的血清中，两种方法呈高度线性相关。与动力学Jaffe反应相比，酶法肌酐检测能有效处理大多数干扰物，但成本更高且保质期更短。
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