Meagher S, McGeachie J, Prendergast F
Ann Surg. 1984 Aug;200(2):153-8. doi: 10.1097/00000658-198408000-00006.
Iliolumbar vein to iliac artery grafts were placed in 21 rats by microsurgical techniques. Graft innervation was examined at five time intervals between 1 and 32 weeks after surgery. Nerve fibers were demonstrated microscopically by formaldehyde-induced fluorescence of catecholamines. The morphology and degree of graft innervation were assessed, semiquantitatively, relative to the contralateral iliac artery (control) within each animal. Nerves were seen in the graft region as early as 2 weeks, but it was not until 4 weeks that they were present along its length (5 mm). The formation of a nerve plexus in the adventitia surrounding the graft was evident at 8 weeks. By 16 weeks the degree of innervation in the graft had increased to a level that was greater than the control iliac artery in three of four animals examined. Grafts at 32 weeks were also hyperinnervated. However, the morphology of this innervation was different from the control arteries; nerve fibers were finer, not varicosed, and were located at a greater distance from the outer layer of smooth muscle cells. The origin of the nerves appeared to be collateral sprouts from nerves supplying the adjacent iliac vein and also from invading vasa vasorum. The host iliac artery nerve plexus did not contribute to graft innervation.
采用显微外科技术将髂腰静脉-髂动脉移植物植入21只大鼠体内。在术后1至32周的五个时间间隔对移植物神经支配情况进行检查。通过儿茶酚胺的甲醛诱导荧光在显微镜下显示神经纤维。相对于每只动物对侧的髂动脉（对照），对移植物神经支配的形态和程度进行半定量评估。早在2周时就在移植物区域看到神经，但直到4周时神经才沿其全长（5毫米）分布。8周时移植物周围外膜中神经丛的形成明显可见。到16周时，在所检查的四只动物中的三只中，移植物的神经支配程度已增加到高于对照髂动脉的水平。32周时的移植物也存在神经支配过度的情况。然而，这种神经支配的形态与对照动脉不同；神经纤维更细，无静脉曲张，且距平滑肌细胞外层的距离更远。神经的起源似乎是供应相邻髂静脉的神经的侧支芽以及侵入的血管滋养管。宿主髂动脉神经丛对移植物神经支配没有贡献。
