Weicker H, Feraudi M, Hägele H, Pluto R
Clin Chim Acta. 1984 Aug 15;141(1):17-25. doi: 10.1016/0009-8981(84)90162-1.
The catecholamines in plasma and urine were determined by electrochemical detection after separation on a HPLC column RCM 100 C 18. Linear calibration plots of epinephrine, norepinephrine and dopamine have been obtained in the range expected to appear in urine or plasma. Coefficients of variation (0.5 to 10%), recovery rates (60 to 80%) and the standard deviation were satisfactory, whereas plasma dopamine showed a greater variation (10-15%). The values ascertained by this technique were compared with those determined by the radioenzymatic assay of Da Prada et al. They showed a good correlation for norepinephrine r = 0.92, p less than 0.001 and epinephrine r = 0.80, p less than 0.01, but less for dopamine r = 0.1. Experimental details of isolation from plasma with Al2O3 adsorption and desorption with HClO4, and from urine with ion exchange chromatography on Bio-Rex 70 and elution with boric acid are described. Technical modifications which improve the method are reported. The optimized assay could reliably be employed in investigations of more than 3000 urine and plasma samples obtained from patients and athletes before and after exercise.
血浆和尿液中的儿茶酚胺通过在HPLC柱RCM 100 C 18上分离后进行电化学检测来测定。在预期出现在尿液或血浆中的范围内，已获得肾上腺素、去甲肾上腺素和多巴胺的线性校准曲线。变异系数（0.5%至10%）、回收率（60%至80%）和标准差令人满意，而血浆多巴胺的变异较大（10% - 15%）。将该技术确定的值与Da Prada等人通过放射酶法测定的值进行了比较。结果显示，去甲肾上腺素的相关性良好，r = 0.92，p < 0.001；肾上腺素的相关性为r = 0.80，p < 0.01；但多巴胺的相关性较差，r = 0.1。描述了用Al2O3吸附从血浆中分离以及用高氯酸解吸，以及用Bio-Rex 70进行离子交换色谱法从尿液中分离并用硼酸洗脱的实验细节。报告了改进该方法的技术修改。优化后的检测方法可可靠地用于对3000多个从患者和运动员运动前后采集的尿液和血浆样本的研究。
