Paz G F, Homonnai Z T
Contraception. 1984 Jun;29(6):543-52. doi: 10.1016/s0010-7824(84)80016-5.
Gossypol acetic acid is a polyphenolic compound present in the seed of cotton plants. Its antifertility activity by inhibition of spermatogenesis was proven in a large group of animals, including man. In the present study, the direct effect of gossypol acetic acid on collagenase isolated rat I-cells (interstitial cells) was investigated. It was shown that gossypol acetic acid depressed significantly the metabolic rate of the cells. Glucose utilization was abolished by a starting dose of 100 micrograms/ml. Oxygen consumption of I-cells was reduced even at a smaller dose of gossypol (50 micrograms/ml). At these doses, the vitality of the cells remained (proven by trypan blue exclusion test). 3 beta-Hydroxysteroid dehydrogenase (3 beta-HSD) histochemical stain was slightly decreased. Increasing doses of gossypol caused a marked decrease in the vitality of I-cells and a dramatic drop in histochemical stain for 3 beta-HSD. The pH of the medium was not changed at any dose of treatment. In cultures of I-cells not stimulated by hCG, gossypol did not affect the tonic slow release of testosterone. Thus, gossypol acetic acid has a direct inhibitory effect on isolated rat I-cells, depressing cell metabolism. The failure of some of the other groups to show such an effect, especially in vivo, can be attributed to differences in the dose of treatment and strain of animals.
醋酸棉酚是一种存在于棉花植物种子中的多酚类化合物。其通过抑制精子发生的抗生育活性已在包括人类在内的大量动物中得到证实。在本研究中，研究了醋酸棉酚对分离的大鼠I细胞（间质细胞）胶原酶的直接作用。结果表明，醋酸棉酚显著降低了细胞的代谢率。起始剂量为100微克/毫升时，葡萄糖利用被消除。即使在较低剂量的棉酚（50微克/毫升）下，I细胞的耗氧量也会降低。在这些剂量下，细胞活力仍然存在（通过台盼蓝排斥试验证实）。3β-羟基类固醇脱氢酶（3β-HSD）组织化学染色略有下降。棉酚剂量增加导致I细胞活力显著下降，3β-HSD组织化学染色急剧下降。在任何治疗剂量下，培养基的pH值均未改变。在未受hCG刺激的I细胞培养物中，棉酚不影响睾酮的持续性缓慢释放。因此，醋酸棉酚对分离的大鼠I细胞具有直接抑制作用，可降低细胞代谢。其他一些组未能显示出这种作用，尤其是在体内，可归因于治疗剂量和动物品系的差异。
