Kim S U, Moretto G, Ruff B, Shin D H
Acta Neuropathol. 1984;64(2):172-5. doi: 10.1007/BF00695582.
Human adult oligodendrocytes were bulk-isolated from the brains obtained at autopsy by the Percoll density gradient method and cultured in vitro for 2-28 days. Cultured human oligodendrocytes were then scraped from the dishes or coverslips, frozen at -70 degrees C in the presence of 10% dimethyl sulfoxide as a cryoprotectorant, and then stored in liquid nitrogen tank. After the fast thawing, 55%-70% of human oligodendrocytes plus astrocytes were found to survive in the culture. Identification of cell types was established by using antibodies directed against galactocerebroside (oligodendrocyte) and glial fibrillary acidic protein (astrocyte).
通过Percoll密度梯度法从尸检获得的大脑中批量分离出成人人类少突胶质细胞，并在体外培养2 - 28天。然后将培养的人类少突胶质细胞从培养皿或盖玻片上刮下，在含有10%二甲基亚砜作为冷冻保护剂的条件下于-70℃冷冻，随后储存在液氮罐中。快速解冻后，发现培养物中有55% - 70%的人类少突胶质细胞和星形胶质细胞存活。通过使用针对半乳糖脑苷脂（少突胶质细胞）和胶质纤维酸性蛋白（星形胶质细胞）的抗体来鉴定细胞类型。
