Smith A L, Paturzo F X, Gardner E P, Morgenstern S, Cameron G, Wadley H
Can J Comp Med. 1984 Jul;48(3):335-7.
Two epizootics of lymphocytic choriomeningitis virus in mice occurred within two months in one research facility consisting of several widely separated rooms. These outbreaks developed despite intensive institutional monitoring policies designed to prevent introduction and spread of lymphocytic choriomeningitis virus. Evidence derived from serological and virological assays and interviews with the concerned investigators suggested that a single transplantable tumor carried in mice may have been responsible for spread of the virus. However, the tumor was not contaminated with lymphocytic choriomeningitis virus at the time of its introduction into the mouse facility. The origin of the virus responsible for the outbreaks was not definitively established although data supported an hypothesis that the virus was introduced into the research facility by a wild or feral mouse. Virus spread from infected mice to humans did not occur, as measured by serological tests. However, a large and valuable animal facility was depopulated for safety reasons. Absorption of sera with lymphocytic choriomeningitis virus antigen proved a necessary and reliable method for confirming specificity of lymphocytic choriomeningitis virus fluorescence-positive reactions.
在一个由几个分布广泛的房间组成的研究机构中，两个月内小鼠发生了两起淋巴细胞性脉络丛脑膜炎病毒疫情。尽管该机构制定了旨在防止淋巴细胞性脉络丛脑膜炎病毒传入和传播的强化监测政策，但这些疫情仍有发生。血清学和病毒学检测以及与相关研究人员的访谈证据表明，小鼠携带的一种可移植肿瘤可能是病毒传播的原因。然而，该肿瘤在引入小鼠设施时并未被淋巴细胞性脉络丛脑膜炎病毒污染。尽管数据支持病毒是由野生或野生小鼠引入研究机构的假设，但引发疫情的病毒来源尚未明确确定。通过血清学检测，未发现病毒从感染小鼠传播至人类。然而，出于安全考虑，一个规模庞大且价值高昂的动物设施被清空。用淋巴细胞性脉络丛脑膜炎病毒抗原吸收血清被证明是确认淋巴细胞性脉络丛脑膜炎病毒荧光阳性反应特异性的一种必要且可靠的方法。
