细胞培养中的分离与传统及改良小鼠抗体产生试验用于检测鼠类病毒的比较。
Comparison of isolation in cell culture with conventional and modified mouse antibody production tests for detection of murine viruses.
作者信息
de Souza M, Smith A L
机构信息
Section of Comparative Medicine, Yale University School of Medicine, New Haven, Connecticut 06510.
出版信息
J Clin Microbiol. 1989 Jan;27(1):185-7. doi: 10.1128/jcm.27.1.185-187.1989.
Abstract
The sensitivity of the mouse antibody production test with intraperitoneal or intrasplenic inoculation of mice with reovirus type 3, minute virus of mice, lymphocytic choriomeningitis virus, or mouse hepatitis virus was compared with that of direct isolation in cultured cells. The mouse antibody production test for detection of mouse hepatitis virus was significantly more sensitive than virus isolation in permissive cells, but differences in sensitivity were less marked for the other three viruses. The intrasplenic route of inoculation did not yield seroconversion that occurred earlier or more consistently than that detected after intraperitoneal inoculation.
摘要
将小鼠腹腔内或脾内接种呼肠孤病毒3型、小鼠微小病毒、淋巴细胞性脉络丛脑膜炎病毒或小鼠肝炎病毒后的小鼠抗体产生试验的敏感性，与在培养细胞中直接分离病毒的敏感性进行了比较。检测小鼠肝炎病毒的小鼠抗体产生试验比在允许性细胞中进行病毒分离的敏感性显著更高，但对于其他三种病毒，敏感性差异不那么明显。脾内接种途径产生的血清转化并不比腹腔接种后检测到的血清转化更早或更稳定。
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