van Oost B A, van den Beld B, Cloin L G, Marx J J
Clin Biochem. 1984 Aug;17(4):263-9. doi: 10.1016/s0009-9120(84)90189-9.
A sandwich-type radioimmunoassay for serum ferritin was developed using iron-rich human liver ferritin and evaluated for its clinical usefulness. In young healthy males and females, the mean serum ferritin concentrations were 44 micrograms/L (range 7-158) and 16 micrograms/L (range 4-56), respectively. In anemic patients lower serum ferritin concentrations were found, while in most patients with iron overload serum ferritin concentrations well above 1000 micrograms/L were measured. Comparison of our method with a commercially available radioimmunoassay kit revealed a good correlation, except for sera with very low ferritin concentrations. Comparison with serum iron and transferrin parameters in patients with iron deficiency demonstrated that serum ferritin concentrations might be subnormal in a majority of patients with otherwise normal iron indices. Up to 70% of the ferritin in serum of normal subjects could bind to concanavalin A-Sepharose, indicating its glycoprotein nature. It is concluded that our serum ferritin radioimmunoassay gave reliable results and was useful in the laboratory diagnosis of latent iron-deficiency and in the analysis of the heterogeneity of serum ferritin.
采用富含铁的人肝脏铁蛋白建立了一种夹心型血清铁蛋白放射免疫测定法，并对其临床实用性进行了评估。在年轻健康男性和女性中，血清铁蛋白平均浓度分别为44微克/升（范围7 - 158）和16微克/升（范围4 - 56）。在贫血患者中发现血清铁蛋白浓度较低，而在大多数铁过载患者中，测得的血清铁蛋白浓度远高于1000微克/升。除了铁蛋白浓度极低的血清外，我们的方法与市售放射免疫测定试剂盒的比较显示出良好的相关性。与缺铁患者的血清铁和转铁蛋白参数比较表明，在大多数铁指标正常的患者中，血清铁蛋白浓度可能低于正常水平。正常受试者血清中高达70%的铁蛋白可与伴刀豆球蛋白A - 琼脂糖结合，表明其糖蛋白性质。结论是，我们的血清铁蛋白放射免疫测定法给出了可靠的结果，可用于潜在缺铁的实验室诊断以及血清铁蛋白异质性分析。
