Bayer H, Gruber W, Schneider J, Hunsmann G
EMBO J. 1984 Aug;3(8):1925-30. doi: 10.1002/j.1460-2075.1984.tb02069.x.
Using terminal position, hydrophilicity, predicted reverse turns and type specificity as criteria, five oligopeptides were selected for synthesis from the amino acid sequence of the envelope glycopolypeptide gp70 of Friend murine leukaemia virus. These peptides corresponded to the amino acids 6-12 (pep1), 124-131 (pep2), 256-262 (pep3), 283-290 (pep4) and 434-441 (pep5). After coupling to carriers, bovine serum albumin or keyhole limpet hemocyanin, antisera were prepared in rabbits. All of the five oligopeptides were immunogenic and pep1, pep2, pep4 and pep5 were able to elicit antibodies to the native glycopolypeptide. These sequence-specific antisera distinguished between glycoproteins of different leukaemia viruses. At least three of the selected peptides, the type-specific oligopeptides pep3, pep4 and pep5, were found to be natural epitopes of gp70.
以末端位置、亲水性、预测的反向转角和类型特异性为标准，从弗氏小鼠白血病病毒包膜糖多肽gp70的氨基酸序列中选择了5种寡肽进行合成。这些肽对应于氨基酸6 - 12（肽1）、124 - 131（肽2）、256 - 262（肽3）、283 - 290（肽4）和434 - 441（肽5）。与载体牛血清白蛋白或钥孔血蓝蛋白偶联后，在兔体内制备抗血清。所有5种寡肽都具有免疫原性，肽1、肽2、肽4和肽5能够引发针对天然糖多肽的抗体。这些序列特异性抗血清能够区分不同白血病病毒的糖蛋白。发现所选的至少三种肽，即类型特异性寡肽肽3、肽4和肽5，是gp70的天然表位。
