通过高效液相色谱法分离人巯基白蛋白和非巯基白蛋白。
Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography.
作者信息
Sogami M, Nagoka S, Era S, Honda M, Noguchi K
出版信息
Int J Pept Protein Res. 1984 Aug;24(2):96-103. doi: 10.1111/j.1399-3011.1984.tb00933.x.
Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the second one to human nonmercaptalbumin (HNA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak on PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.
在PGP 2000柱（0.10 M磷酸钠缓冲液，0.30 M氯化钠，pH 6.86）上对人血清白蛋白（HSA）进行凝胶排阻高效液相色谱（HPLC）分析显示至少有两个峰，主要成分对应于人巯基白蛋白（HMA），第二个峰对应于人非巯基白蛋白（HNA）。HMA和HNA分离的机制可能是由于树脂与HSA的相互作用较弱。在PGP 2000柱上对牛血浆白蛋白（BPA）进行HPLC分析显示为单峰。HSA的洗脱体积大于BPA的洗脱体积，从而实现了HSA和BPA的清晰分离。
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