Haralabidis S T
Ann Trop Med Parasitol. 1984 Jun;78(3):295-300. doi: 10.1080/00034983.1984.11811819.
Specific antibodies against Giardia lamblia in 92 sera from asymptomatic carriers were investigated by ELISA. Using cyst antigen, antibodies were detected in 91 of the sera, by means of alkaline phosphatase-labelled antispecies immunoglobulin. The E/405nm values of the positive sera ranged between 0.350 and 1.700, whereas in the 78 control sera they were 0.270 +/- 0.220. Cross-reactivity between the anti-G. lamblia antibodies and 18 heterologous parasitic antigens and fractions was also studied. Cross-reactions were observed with antigens of Toxoplasma gondii, Sarcocystis spp., Leishmania donovani, Fasciola hepatica Y, F. hepatica I, Echinococcus granulosus AM, Cysticercus tenuicollis, Taenia saginata, Dipylidium caninum Y, D. caninum I, Trichuris suis Y, T. suis I, Trichinella spiralis and Litomosoides carinii. No cross-reactions were observed with the antigens and fractions of Entamoeba histolytica, Dicrocoelium dendriticum, Schistosoma mansoni and Echinococcus granulosus Y. The results show that the ELISA could be easily and very successfully applied for routine diagnosis of giardiasis.
采用酶联免疫吸附测定法（ELISA）检测了92份无症状携带者血清中抗蓝氏贾第鞭毛虫的特异性抗体。使用包囊抗原，借助碱性磷酸酶标记的抗种免疫球蛋白在91份血清中检测到了抗体。阳性血清的E/405nm值在0.350至1.700之间，而78份对照血清的该值为0.270±0.220。还研究了抗蓝氏贾第鞭毛虫抗体与18种异源寄生虫抗原及组分之间的交叉反应。观察到与刚地弓形虫、肉孢子虫属、杜氏利什曼原虫、肝片吸虫Y、肝片吸虫I、细粒棘球绦虫AM、细颈囊尾蚴、牛带绦虫、犬复孔绦虫Y、犬复孔绦虫I、猪鞭虫Y、猪鞭虫I、旋毛虫和卡里尼丝虫的抗原发生交叉反应。未观察到与溶组织内阿米巴、枝双腔吸虫、曼氏血吸虫和细粒棘球绦虫Y的抗原及组分发生交叉反应。结果表明，ELISA可轻松且非常成功地应用于贾第虫病的常规诊断。
