用于检测粪便样本中艰难梭菌毒素的乳胶凝集试验。
Latex agglutination test for detection of Clostridium difficile toxin in stool samples.
作者信息
Shahrabadi M S, Bryan L E, Gaffney D, Coderre S E, Gordon R, Pai C H
出版信息
J Clin Microbiol. 1984 Sep;20(3):339-41. doi: 10.1128/jcm.20.3.339-341.1984.
Abstract
A total of 163 stool specimens were tested for detection of Clostridium difficile and its toxin by cytotoxicity assay with tissue culture, latex agglutination test, and isolation of the organism. From 33 specimens which were positive for toxin by cytotoxicity, 30 were positive by the latex agglutination test; the organism was isolated from 21. The total number of samples which were positive with the latex agglutination test was 44. The predictive value of a positive latex agglutination result relative to the cytotoxicity test was 68%, and the predictive value of a negative result was 97.5%. The specificity and sensitivity of the latex agglutination test relative to the cytotoxicity assay and the low cost and simple facilities required indicate that the latex agglutination test is a useful procedure for screening for C. difficile toxins, provided that positive latex results are confirmed by cytotoxicity assay.
摘要
通过组织培养细胞毒性试验、乳胶凝集试验以及该微生物的分离，共检测了163份粪便标本以检测艰难梭菌及其毒素。在33份经细胞毒性试验毒素呈阳性的标本中，30份经乳胶凝集试验呈阳性；从21份标本中分离出了该微生物。乳胶凝集试验呈阳性的样本总数为44份。乳胶凝集试验阳性结果相对于细胞毒性试验的预测值为68%，阴性结果的预测值为97.5%。乳胶凝集试验相对于细胞毒性试验的特异性和敏感性，以及所需的低成本和简单设备表明，乳胶凝集试验是一种筛查艰难梭菌毒素的有用方法，前提是乳胶试验阳性结果需经细胞毒性试验确认。
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