通过毒素检测快速鉴定艰难梭菌。
Rapid identification of Clostridium difficile by toxin detection.
作者信息
Chang T W, Gorbach S L
出版信息
J Clin Microbiol. 1982 Mar;15(3):465-7. doi: 10.1128/jcm.15.3.465-467.1982.
Abstract
Rapid identification of Clostridium difficile in a stool specimen could be accomplished within 24 h by detection of toxin elaborated in an agar or broth culture containing cycloserine and cefoxitin. Broth culture seemed to give a more rapid and sensitive result than the agar plate culture. For cultivation of C. difficile in stool, we recommend the use of chopped meat broth and blood agar plate, the former for toxin detection in 1 to 2 days and the latter for colonial morphology and isolation of a pure culture.
摘要
通过检测在含有环丝氨酸和头孢西丁的琼脂或肉汤培养物中产生的毒素，可在24小时内快速鉴定粪便标本中的艰难梭菌。肉汤培养似乎比琼脂平板培养给出的结果更快且更灵敏。对于粪便中艰难梭菌的培养，我们推荐使用碎肉肉汤和血琼脂平板，前者用于1至2天内的毒素检测，后者用于观察菌落形态和分离纯培养物。
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Scand J Infect Dis Suppl. 1980(Suppl 22):37-40.
2
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3
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