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α-六氯环己烷的生物降解。VII. 大鼠肝细胞溶质中α-六氯环己烷脱氯酶的拆分、纯化及特性鉴定

Biodegradation of alpha-hexachlorocyclohexane. VII. Resolution, purification, and characterization of an alpha-HCH dechlorinating enzyme from rat liver cytosol.

作者信息

Kraus P

出版信息

Naunyn Schmiedebergs Arch Pharmacol. 1976 Dec;296(1):67-72. doi: 10.1007/BF00498841.

DOI:10.1007/BF00498841
PMID:64937
Abstract

From rat liver cytosol, an enzyme was isolated which catalyzes the dechlorination of alpha-, gamma-, and delta-HCH. The enzyme also catalyzes the conjugation of GSH with 1,2-dichloro-4-nitrobenzene, and with 1 -chloro-2,4-dinitrobenzene. The enzyme has a molecular weight of about 46000 and its molecule is composed of two subunits of similar size. The optimum for the dechlorination of alpha-HCH lies at pH 8.0. The Michaelis constant is 0.12 mM for alpha-HCH (with 1 mM GSH as constant substrate), and maximal velocity was determined to be 0.25 moles Cl- -min -1 per mole enzyme.

摘要

从大鼠肝脏细胞溶质中分离出一种酶，它能催化α-、γ-和δ-六氯环己烷的脱氯反应。该酶还能催化谷胱甘肽与1,2-二氯-4-硝基苯以及与1-氯-2,4-二硝基苯的结合反应。该酶的分子量约为46000，其分子由两个大小相似的亚基组成。α-六氯环己烷脱氯反应的最适pH值为8.0。以1 mM谷胱甘肽作为固定底物时，α-六氯环己烷的米氏常数为0.12 mM，测得最大反应速度为每摩尔酶每分钟0.25摩尔氯离子。

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本文引用的文献

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An enzyme from rat liver catalysing conjugations with glutathione.一种来自大鼠肝脏的可催化与谷胱甘肽结合反应的酶。
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
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Biochem J. 1973 Dec;135(4):797-804. doi: 10.1042/bj1350797.
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Cross specificity in some vertebrate and insect glutathione-transferases with methyl parathion (dimethyl p-nitrophenyl phosphorothionate), 1-chloro-2,4-dinitro-benzene and s-crotonyl-N-acetylcysteamine as substrates.一些脊椎动物和昆虫谷胱甘肽转移酶以甲基对硫磷（二甲基对硝基苯基硫代磷酸酯）、1-氯-2,4-二硝基苯和反式巴豆酰-N-乙酰半胱氨酸为底物时的交叉特异性。
Biochem J. 1973 Nov;135(3):385-92. doi: 10.1042/bj1350385.
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Mercapturic acid formation: the several glutathione transferases of rat liver.巯基尿酸的形成：大鼠肝脏中的几种谷胱甘肽转移酶。
Biochem Biophys Res Commun. 1973 Jun 19;52(4):1123-8. doi: 10.1016/0006-291x(73)90616-5.
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Enzymatic conjugation of epoxides with glutathione.环氧化物与谷胱甘肽的酶促共轭反应。
J Biol Chem. 1973 May 25;248(10):3702-7.
9
A new method for determining the Michaelis constant.一种测定米氏常数的新方法。
Biochem J. 1974 Oct;143(1):93-5. doi: 10.1042/bj1430093.
10
Glutathione S-transferase A. A novel kinetic mechanism in which the major reaction pathway depends on substrate concentration.谷胱甘肽S-转移酶A。一种新的动力学机制，其中主要反应途径取决于底物浓度。
J Biol Chem. 1974 Nov 25;249(22):7140-7.