Slomiany A, Yano S, Slomiany B L, Glass G B
J Biol Chem. 1978 Jun 10;253(11):3785-91.
The lipid composition of the "mucous barrier" of rat stomach was investigated. Cellular mucus of the mucous cells from gastric epithelium and surface mucus from gastric mucosa were obtained by perfusion in vivo of Ghosh-Lai rat stomachs with 2 M NaCl. Lipids extracted from dialyzed and lyophilized 2 M NaCl perfusates and 0.9% NaCl (saline) controls were quantitatively separated into single components by means of two-dimensional thin layer chromatography and compared. Significant differences in the level of cholesterol esters, di- and triglycerides, and glycolipids were found. Three to 4 times greater quantities of cholesterol esters and di- and triglycerides, and 2 to 6 times greater quantities of glycolipids were found in 2 M NaCl perfusates as compared to saline controls. The glycolipid fraction consisted of neutral and acidic glyceroglucolipids and was devoid of glycosphingolipids. The phospholipids constituted the smallest portion of the lipids present in 2 M NaCl and saline perfusates and were 2 times higher in 2 M NaCl. These data indicate that mucous barrier in addition to mucins contains considerable quantities of lipids of which glyceroglucolipids are the most prominent components.
对大鼠胃“黏液屏障”的脂质组成进行了研究。通过用2M NaCl对Ghosh-Lai大鼠胃进行体内灌注，获取胃上皮黏液细胞的细胞黏液和胃黏膜表面黏液。从透析并冻干的2M NaCl灌注液和0.9% NaCl（生理盐水）对照中提取的脂质，通过二维薄层色谱法定量分离为单一成分并进行比较。发现胆固醇酯、甘油二酯和甘油三酯以及糖脂水平存在显著差异。与生理盐水对照相比，在2M NaCl灌注液中发现的胆固醇酯、甘油二酯和甘油三酯量多3至4倍，糖脂量多2至6倍。糖脂部分由中性和酸性甘油葡糖脂组成，不含糖鞘脂。磷脂在2M NaCl和生理盐水灌注液中的脂质中占比最小，在2M NaCl中高出2倍。这些数据表明，黏液屏障除了黏蛋白外还含有大量脂质，其中甘油葡糖脂是最主要的成分。
