免疫黏附血凝反应：补体结合血清学的替代方法。
Immune adherence hemagglutination: alternative to complement-fixation serology.
作者信息
Lennette E T, Lennette D A
出版信息
J Clin Microbiol. 1978 Mar;7(3):282-5. doi: 10.1128/jcm.7.3.282-285.1978.
Abstract
Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.
摘要
采用标准程序，将免疫黏附血凝试验（IAHA）与补体结合试验（CF）进行比较，以使用多种市售抗原对人血清进行血清学检测。这些抗原包括单纯疱疹病毒、麻疹病毒、巨细胞病毒和B型流感病毒，以及肺炎支原体和鹦鹉热衣原体（衣原体属）。结果发现，IAHA试验与CF试验一样具有特异性，但对所有检测抗原的敏感性要高4至20倍。IAHA方法的抗原效价也更高，完成试验所需时间，IAHA方法仅为4小时，而CF方法为20小时。IAHA试验敏感性的提高应使其可用于免疫测定以及近期感染的血清学诊断。
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