通过免疫粘连血凝反应检测抗EB病毒衣壳抗原的抗体。
Detection of antibodies to Epstein-Barr virus capsid antigen by immune adherence hemagglutination.
作者信息
Lennette E T, Ward E, Henle G, Henle W
出版信息
J Clin Microbiol. 1982 Jan;15(1):69-73. doi: 10.1128/jcm.15.1.69-73.1982.
Abstract
The immune adherence hemagglutination assay was found to be as sensitive and specific as the indirect immunofluorescence technique for titration of antibodies to Epstein-Barr virus capsid antigen. Satisfactory virus capsid antigen-specific and negative control antigens for the immune adherence hemagglutination assay were prepared from cell extracts of the Epstein-Barr virus producer P3HR-1 and the Epstein-Barr virus genome-negative BJAB lymphoblastoid cell lines, respectively. As the immune adherence hemagglutination assay can be used to titrate antibodies to both the heterophil antigen of the Paul-Bunnell type and to virus capsid antigen, it offers a promising alternative to the immunofluorescence methods in the serodiagnosis of Epstein-Barr virus infections which can be performed by most diagnostic laboratories.
摘要
免疫黏附血凝试验被发现对于滴定抗爱泼斯坦-巴尔病毒衣壳抗原的抗体而言，与间接免疫荧光技术一样灵敏且特异。用于免疫黏附血凝试验的令人满意的病毒衣壳抗原特异性和阴性对照抗原，分别由爱泼斯坦-巴尔病毒产生株P3HR-1和爱泼斯坦-巴尔病毒基因组阴性的BJAB淋巴母细胞系的细胞提取物制备而成。由于免疫黏附血凝试验可用于滴定针对保罗-邦内尔型嗜异性抗原和病毒衣壳抗原的抗体，它为大多数诊断实验室可开展的爱泼斯坦-巴尔病毒感染血清学诊断中的免疫荧光方法提供了一种有前景的替代方法。
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