Krakowka S, Wallace A L, Koestner A
J Clin Microbiol. 1978 Mar;7(3):292-7. doi: 10.1128/jcm.7.3.292-297.1978.
A simple and rapid (24-h) assay for peripheral blood lymphocyte-associated immunity to canine distemper virus (CDV) is described. The test is based upon leukocyte-associated inhibition of CDV-induced syncytia formation in Vero cells. The technique quantitates the response morphologically, thereby eliminating the requirement for release of radiolabeled compounds. Positive results were determined from specific-pathogen-free and gnotobiotic dogs exposed to CDV via hyperimmunization, vaccination with a modified live virus vaccine, and after virulent virus infection. Preinoculation lymphocytes and lymphocytes from non-immune dogs did not inhibit CDV-induced syncytia formation. Maximum responses were observed 7 to 21 days after initial exposure and declined thereafter. The method can be used to further investigate the role of immune lymphocytes in the recovery from CDV infection.
本文描述了一种用于检测外周血淋巴细胞对犬瘟热病毒（CDV）相关免疫的简单快速（24小时）检测方法。该检测基于白细胞对Vero细胞中CDV诱导的合胞体形成的相关抑制作用。该技术通过形态学方法对反应进行定量，从而无需释放放射性标记化合物。通过超免疫、接种改良活病毒疫苗以及感染强毒病毒等方式使无特定病原体犬和悉生动物犬接触CDV后，获得了阳性结果。接种前的淋巴细胞以及来自非免疫犬的淋巴细胞均未抑制CDV诱导的合胞体形成。初次接触后7至21天观察到最大反应，此后反应下降。该方法可用于进一步研究免疫淋巴细胞在CDV感染恢复过程中的作用。
