Appel M J, Shek W R, Shesberadaran H, Norrby E
Arch Virol. 1984;82(1-2):73-82. doi: 10.1007/BF01309369.
Pairs of specific pathogen free dogs were immunized with two injections of heat inactivated canine distemper virus (CDV) or one injection of a live CDV or live measles virus (MV) vaccine. Three unimmunized dogs were used as controls. All 9 dogs were challenged with virulent CDV (Snyder Hill strain). The three unimmunized dogs developed severe signs of disease with a lethal infection in one. The two dogs immunized with live CDV vaccine developed a strong humoral as well as cellular immune response after immunization and were protected against virus replication. Animals immunized with either inactivated CDV or modified live MV failed to develop a measurable cellular immune response after immunization and had a comparatively weak humoral immune response to distemper antigens. They showed mild signs of infection after challenge and responded with strong anamnestic cellular and humoral immunity. The measles vaccine immunized dogs had a moderate serum titer of measles hemolysin-inhibiting antibodies which, after exposure to distemper virus, was boosted to high levels. It is proposed that this response plays a role in the mitigation of the virulent distemper infection in these animals.
将特定病原体清除的犬只配对，用两剂热灭活犬瘟热病毒（CDV）或一剂活CDV或活麻疹病毒（MV）疫苗进行免疫。三只未免疫的犬只用作对照。所有9只犬只均用强毒CDV（斯奈德·希尔株）进行攻击。三只未免疫的犬只出现严重疾病症状，其中一只发生致死性感染。两只用活CDV疫苗免疫的犬只在免疫后产生了强烈的体液免疫和细胞免疫反应，并受到保护免受病毒复制。用灭活CDV或改良活MV免疫的动物在免疫后未能产生可测量的细胞免疫反应，并且对犬瘟热抗原的体液免疫反应相对较弱。它们在攻击后表现出轻微的感染症状，并以强烈的回忆性细胞免疫和体液免疫做出反应。接种麻疹疫苗的犬只具有中等水平的麻疹溶血抑制抗体血清滴度，在接触犬瘟热病毒后，该滴度升高至高水平。有人提出，这种反应在减轻这些动物的强毒犬瘟热感染中起作用。
