Krakowka S, Mador R A, Koestner A
Acta Neuropathol. 1978 Sep 15;43(3):235-41. doi: 10.1007/BF00691584.
Gnotobiotic puppies were intraperitoneally infected with R252-canine distemper virus (CDV), and treated with normal serum (NS) or CDV-specific antibody. Resultant central nervous system (CNS) lesions were evaluated by light and immunofluorescence microscopy. Lesions in NS-treated dogs, if present, consisted of small foci of neuronal degeneration and accompanying microgliosis, restricted to grey matter of the frontal lobe. Dogs receiving anti-CDV antibody, in contrast, had extensive lesions in the brain. Laminar cortical necrosis, gliosis and astrocytic activation of the grey matter of the frontal lobe was the most consistent lesion observed. In addition, foci of cerebellar granule cell layer degeneration and necrosis was seen along with segmental poliomalacia of the spinal cord. In several dogs, eosinophilic intracytoplasmic and intranuclear viral inclusion bodies were observe in neurons. The lesions observed in these dogs are compared to previously reported lesions of CDV infection in dogs.
无菌幼犬经腹腔感染R252 - 犬瘟热病毒（CDV），并分别用正常血清（NS）或CDV特异性抗体进行治疗。通过光学显微镜和免疫荧光显微镜对由此产生的中枢神经系统（CNS）病变进行评估。接受NS治疗的犬只若出现病变，表现为局限于额叶灰质的小灶性神经元变性及伴随的小胶质细胞增生。相比之下，接受抗CDV抗体治疗的犬只脑部出现广泛病变。额叶灰质的层状皮质坏死、胶质细胞增生和星形细胞活化是最常见的病变。此外，还可见小脑颗粒细胞层变性和坏死灶，以及脊髓节段性软化。在几只犬只的神经元中观察到嗜酸性胞浆内和核内病毒包涵体。将这些犬只中观察到的病变与先前报道的犬只CDV感染病变进行比较。
