Dunn S, Jester J V, Arthur J, Smith R E
Arch Ophthalmol. 1984 Nov;102(11):1666-70. doi: 10.1001/archopht.1984.01040031350025.
The effect of radial keratotomy on the corneal endothelium of subhuman primates was studied by serial specular microscopy and histopathology. Fixed-frame analysis of specular micrographs of 13 operated eyes at one month after surgery revealed an endothelial cell loss of 13% (+/- 4%). Follow-up of all 13 eyes for six months disclosed no statistically significant progressive endothelial cell loss. Similarly, no significant loss could be demonstrated in five eyes followed up for 18 months and two eyes followed up four years after surgery. Variable frame analyses of specular micrographs of all 13 unoperated eyes followed up for six months also did not suggest progressive endothelial cell loss following radial keratotomy. Histopathology and ultrastructural studies of corneas undergoing radial keratotomy disclosed no abnormalities suggesting ongoing endothelial cell damage. These findings suggest that radial keratotomy produces an acute postoperative endothelial cell loss that is nonprogressive. Much longer term follow-up with larger numbers of subjects is necessary before the ultimate delayed effects of radial keratotomy on the endothelium can be determined.
通过连续镜面显微镜检查和组织病理学研究了放射状角膜切开术对非人灵长类动物角膜内皮的影响。对术后1个月的13只手术眼的镜面显微照片进行固定框架分析，结果显示内皮细胞损失13%（±4%）。对所有13只眼进行6个月的随访，未发现有统计学意义的内皮细胞进行性损失。同样，在术后随访18个月的5只眼和随访4年的2只眼中，也未发现有明显的细胞损失。对随访6个月的所有13只未手术眼的镜面显微照片进行可变框架分析，也未提示放射状角膜切开术后内皮细胞有进行性损失。对接受放射状角膜切开术的角膜进行组织病理学和超微结构研究，未发现提示内皮细胞持续损伤的异常情况。这些发现表明，放射状角膜切开术会导致术后急性内皮细胞损失，但这种损失不会进行性发展。在确定放射状角膜切开术对内皮的最终延迟影响之前，需要对更多受试者进行更长时间的随访。
