Yamaguchi T, Kaufman H E, Fukushima A, Safir A, Asbell P A
Am J Ophthalmol. 1981 Sep;92(3):313-27. doi: 10.1016/0002-9394(81)90519-5.
We performed anterior radial keratotomy on the right eyes of nine rhesus monkeys, making eight incisions of various depths from 0.36 to 0.5 mm with a No. 76-A Beaver blade. After three months, corneal buttons were obtained from the operated on eyes of seven monkeys by penetrating keratoplasty and two monkeys were killed, and both operated and unoperated on eyes were enucleated. We examined the specimens by scanning and transmission electron microscopy. We saw linear protrusions on the posterior cornea beneath and parallel to the radial keratotomy incisions in all cases. There were swollen endothelial cells at the central cornea in three eyes. In eight corneas, we saw damaged endothelial cells with invading inflammatory cells. The control eyes showed no abnormal histologic findings. These findings suggested that deeper incisions cause more endothelial damage, possibly in relation to structural weakness produced in the cornea. We think that cuts in Bowman's membrane and in the stromal tissue may cause corneal stretching, resulting in a continuing process of injury to the endothelial cell layer. There may be long-term, continuing damage to the corneal endothelium after anterior radial keratotomy.
我们对9只恒河猴的右眼进行了放射状角膜切开术，使用76-A号比弗刀片制作了8条深度从0.36毫米至0.5毫米不等的切口。三个月后，通过穿透性角膜移植术从7只猴子的手术眼获取了角膜纽扣组织，处死了2只猴子，并摘除了手术眼和未手术眼。我们通过扫描电子显微镜和透射电子显微镜对标本进行了检查。在所有病例中，我们均在放射状角膜切开术切口下方且与之平行的后角膜上看到了线性突起。三只眼睛的中央角膜内皮细胞出现肿胀。在8只角膜中，我们看到内皮细胞受损并有炎性细胞侵入。对照眼未发现异常组织学表现。这些发现表明，更深的切口会导致更多的内皮损伤，这可能与角膜产生的结构薄弱有关。我们认为，Bowman膜和基质组织中的切口可能会导致角膜拉伸，从而导致内皮细胞层持续受损。放射状角膜切开术后可能会对角膜内皮造成长期的持续性损伤。
