Kronberg H, Zimmer H G, Neuhoff V
Clin Chem. 1984 Dec;30(12 Pt 1):2059-62.
We describe the design principles of a photometric flatbed scanner with a scan area of 250 X 250 mm2, a dynamic range of 4000 gray levels, a signal/noise ratio of 2000/1, a step size of 0.1 mm, and a step frequency of 2000 steps per second. It is controlled by a microcomputer and used to acquire data for quantitative evaluation of gels or chromatograms. The performance of the instrument is demonstrated by the data obtained by scanning gratings, gray step filters, and high-resolution electropherograms.
我们描述了一种光度平板扫描仪的设计原理，其扫描面积为250×250平方毫米，动态范围为4000个灰度级，信噪比为2000/1，步长为0.1毫米，步频为每秒2000步。它由一台微型计算机控制，用于获取数据以对凝胶或色谱图进行定量评估。通过扫描光栅、灰度阶跃滤波器和高分辨率电泳图所获得的数据展示了该仪器的性能。
