乙型肝炎表面抗原血凝试验——试剂制备的新方法。

Haemagglutination assay for HBsAg -- a new approach to reagent preparation.

作者信息

Hopkins R, Watt A D, Ross S

出版信息

J Immunol Methods. 1978;20:35-41. doi: 10.1016/0022-1759(78)90242-9.

Abstract

Human group O rhesus negative erythrocytes were spherocyted and fixed in glutaraldehyde, then coated initially with HBsAg and subsequently with anti-HBs. The resulting reagent was found to agglutinate in the presence of HBsAg positive serum, but not in HBsAg negative serum. The assay was evaluated for blood donor screening (39,962 donations) over a 6-month period, and was compared with Hepatest (RPHA) and AUSRIA-2 (RIA) for the study of two established HBsAg panels.

摘要

人O型恒河猴阴性红细胞经球形化处理后用戊二醛固定，先包被乙肝表面抗原（HBsAg），随后包被抗HBs。结果发现，所得试剂在HBsAg阳性血清存在时发生凝集，而在HBsAg阴性血清中不发生凝集。在6个月期间对该检测方法进行了献血者筛查评估（39962次献血），并与用于两个既定HBsAg检测板研究的Hepatest（反向被动血凝试验）和AUSRIA - 2（放射免疫分析）进行了比较。