Liljemark W F, Bloomquist C G, Uhl L A, Schaffer E M, Wolff L F, Pihlstrom B L, Bandt C L
Infect Immun. 1984 Dec;46(3):778-86. doi: 10.1128/iai.46.3.778-786.1984.
The periodontal status of maxillary first molars in 284 young adults demonstrating near-health to early disease was evaluated, and supragingival and subgingival plaque samples were collected. Plaque samples were processed anaerobically, enumerated microscopically for bacterial morphotypes, and cultivated on various media to enumerate the microflora. Although haemophili were ubiquitous (recovered in 98.5 and 96.2% of the supragingival and subgingival plaque samples, respectively), 50% of the respective samples had proportions of less than or equal to 1.5% and less than or equal to 0.33% total Haemophilus spp. based on total cultivable microflora. To study the distribution of Haemophilus spp., 377 colonies were identified from modified chocolate agar (selective for oral haemophili) from 14 supragingival and corresponding subgingival samples from 14 subjects. The most prevalent species, Haemophilus parainfluenzae, was found in significantly higher proportions, based on total haemophili on modified chocolate agar, in supragingival and subgingival samples from teeth with shallower probing depths (less than or equal to 3.0 mm) versus deeper probing depths (greater than or equal to 3.0 mm). Additional statistically significant findings included Haemophilus segnis in higher proportions in supragingival samples from deeper sites, Haemophilus aphrophilus in higher proportions in subgingival samples from deeper sites, and Haemophilus paraphrophilus in higher proportions in subgingival samples from shallower sites. Scatter diagrams illustrating the bivariate distributions of proportions of haemophili with proportions of dark-pigmented Bacteroides spp., spirochetes, and streptococci demonstrated that high proportions of haemophili were never recovered from sites with high proportions of Bacteroides spp. or spirochetes. All levels of haemophili, however, were recovered from sites with all levels of streptococci. Two potential systems for interpreting haemophili data were hypothesized for predicting periodontal probing depths. There was highly significant agreement between the two systems. Small but statistically significant correlations were found between the gingival index, probing depth, and attachment level, and proportions of total Haemophilus species in the respective samples.
对284名处于近乎健康到早期疾病阶段的年轻成年人上颌第一磨牙的牙周状况进行了评估，并采集了龈上和龈下菌斑样本。菌斑样本进行厌氧处理，通过显微镜对细菌形态类型进行计数，并在各种培养基上培养以计数微生物群落。尽管嗜血菌普遍存在（分别在98.5%和96.2%的龈上和龈下菌斑样本中检出），但基于可培养微生物群落总数，相应样本中分别有50%的嗜血杆菌属比例小于或等于1.5%和小于或等于0.33%。为了研究嗜血杆菌属的分布，从14名受试者的14个龈上样本及相应龈下样本的改良巧克力琼脂（对口腔嗜血菌有选择性）中鉴定出377个菌落。最常见的菌种副流感嗜血杆菌，基于改良巧克力琼脂上的总嗜血菌数，在探诊深度较浅（小于或等于3.0毫米）的牙齿的龈上和龈下样本中所占比例显著高于探诊深度较深（大于或等于3.0毫米）的样本。其他具有统计学意义的发现包括：在较深部位的龈上样本中，迟缓嗜血杆菌比例较高；在较深部位的龈下样本中，嗜沫嗜血杆菌比例较高；在较浅部位的龈下样本中，副嗜沫嗜血杆菌比例较高。说明嗜血菌比例与黑色色素拟杆菌属、螺旋体和链球菌比例的双变量分布的散点图表明，在拟杆菌属或螺旋体比例高的部位从未检出高比例的嗜血菌。然而，在链球菌比例不同的所有部位均检出了不同水平的嗜血菌。为预测牙周探诊深度，假设了两种解释嗜血菌数据的潜在系统。这两种系统之间存在高度显著的一致性。在牙龈指数、探诊深度和附着水平与相应样本中总嗜血杆菌种类比例之间发现了虽小但具有统计学意义的相关性。
