Visser F R
J Assoc Off Anal Chem. 1984 Sep-Oct;67(5):1020-2.
Trichloroacetic acid (TCA) is suggested as a substitute for the metaphosphoric acid/acetic acid (HPO3-HOAc) solvent system in the AOAC microfluorometric determination of vitamin C. Comparative advantages of TCA are easier handling, greater stability, and the approximate 10-fold increase in sensitivity. For both solvent systems, the influence of acid concentration on fluorescence is described. TCA provides maximum fluorescence enhancement at pH 5.5-6.0.
在AOAC微量荧光法测定维生素C中，建议用三氯乙酸（TCA）替代偏磷酸/乙酸（HPO₃-HOAc）溶剂体系。TCA的比较优势在于操作更简便、稳定性更高，且灵敏度提高了约10倍。对于这两种溶剂体系，均描述了酸浓度对荧光的影响。TCA在pH 5.5 - 6.0时能提供最大的荧光增强效果。
