Peterson M W, Busse W W
J Allergy Clin Immunol. 1984 Dec;74(6):787-93. doi: 10.1016/0091-6749(84)90180-5.
Influenza A virus has been demonstrated to enhance superoxide generation and chemoluminescence (CL) in human polymorphonuclear leucoytes (PMNs) under in vitro conditions. Although the mechanisms of virus-enhanced neutrophil activity is not established, calmodulin concentrations are known to increase in some virus-transformed cells. In the following experiments, we evaluated the PMN response to the calmodulin-inhibitor trifluoperazine (TFP) after an incubation with influenza A virus. Human PMNs were isolated from whole blood and were incubated with either influenza A virus (at 50% egg-infective dose per 1 leukocyte) or noninfected allantoic fluid. After incubation with influenza virus, the CL response of isolated PMNs to opsonized zymosan particles was measured. The influenza virus-treated PMNs had a mean (+/- SEM, n = 16) increase in light emission of 59.5 +/- 7.7%. TFP, in concentrations of 6 micron, 8 micron, and 10 microM, inhibition of CL was similar in influenza virus and allantoic fluid-treated neutrophils. These data suggest that, although the influenza A virus enhanced the PMN "respiratory burst" to opsonized zymosan particles, it did not alter the cell response to one calmodulin inhibitor, TFP.
在体外条件下，甲型流感病毒已被证明可增强人多形核白细胞（PMN）中超氧化物的生成和化学发光（CL）。虽然病毒增强中性粒细胞活性的机制尚未明确，但已知在一些病毒转化细胞中钙调蛋白浓度会增加。在以下实验中，我们评估了甲型流感病毒孵育后PMN对钙调蛋白抑制剂三氟拉嗪（TFP）的反应。从全血中分离出人类PMN，并将其与甲型流感病毒（每1个白细胞50%的鸡胚感染剂量）或未感染的尿囊液一起孵育。与流感病毒孵育后，测量分离出的PMN对调理酵母聚糖颗粒的CL反应。经流感病毒处理的PMN的平均（±SEM，n = 16）发光增加了59.5±7.7%。浓度为6微米、8微米和10微摩尔的TFP对流感病毒和尿囊液处理的中性粒细胞中CL的抑制作用相似。这些数据表明，虽然甲型流感病毒增强了PMN对调理酵母聚糖颗粒的“呼吸爆发”，但它并未改变细胞对一种钙调蛋白抑制剂TFP的反应。
