Karmali M A, Williams A, Fleming P C, Krishnan C, Wood M M
J Hyg (Lond). 1984 Oct;93(2):189-96. doi: 10.1017/s0022172400064706.
A method using an ammonia electrode is being developed for investigating the deamination of amino acids and amides by bacteria. Application of this method to Campylobacter jejuni and C. coli has led to the demonstration of D-asparaginase activity in some strains. This has allowed the subdivision of both species into D-asparaginase-positive and -negative biotypes. Even though the method is in the developmental stage, it was found to be generally reproducible and easy to perform. Areas for further improving the procedure have been identified. The ammonia electrode offers the theoretical possibility of investigating the breakdown of any amino acid by bacteria. It thus opens up a new and practical approach for separating species and strains, particularly in those bacterial groups that are difficult to subdivide by conventional means.
正在开发一种使用氨电极的方法来研究细菌对氨基酸和酰胺的脱氨作用。将该方法应用于空肠弯曲菌和大肠弯曲菌，已证明某些菌株具有D-天冬酰胺酶活性。这使得这两个菌种都可细分为D-天冬酰胺酶阳性和阴性生物型。尽管该方法仍处于开发阶段，但已发现其通常具有可重复性且易于操作。已确定了进一步改进该程序的领域。氨电极提供了研究细菌对任何氨基酸分解作用的理论可能性。因此，它为分离菌种和菌株开辟了一种新的实用方法，特别是对于那些难以用传统方法细分的细菌群体。
