动脉溶酶体胆固醇酯水解酶的亲和层析
Affinity chromatography of arterial lysosomal cholesterol ester hydrolase.
作者信息
Dousset J C, Dousset N, Foglietti M J, Douste-Blazy L
出版信息
Biochim Biophys Acta. 1981 May 22;664(2):273-7. doi: 10.1016/0005-2760(81)90050-3.
PMID:7248326
Abstract
The glycoproteic nature of rabbit aortic lysosomal cholesterol ester hydrolase has been demonstrated by affinity chromatography on Concanavalin A-Sepharose. After chromatography, the enzyme lacks synthesizing activity. This activity is restored by addition of deactivated lysosomes containing more endogenous cholesterol. On the other hand, a hypothesis for the activation role of bis(monoacylglyceryl) phosphate is suggested.
摘要
兔主动脉溶酶体胆固醇酯水解酶的糖蛋白性质已通过伴刀豆球蛋白A-琼脂糖亲和层析得以证明。层析后，该酶缺乏合成活性。通过添加含有更多内源性胆固醇的失活溶酶体可恢复此活性。另一方面，有人提出了双（单酰甘油）磷酸酯激活作用的假说。
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