Castenholz A
Scan Electron Microsc. 1984(Pt 3):1343-52.
The subepithelial lymphatics of the rat tongue were examined by scanning electron microscopy in interstitially fixed tissue and corrosion casts. In tissue preparations fixed at low interstitial pressures, the endothelium exhibits broad and flat cells with serrate endothelial borders. In the course of the intercellular border system, simple junctions and overlapping zones occur. The latter have the appearance of pocketings. In tissue fixed at high interstitial pressures of up to 75 mm Hg, many endothelial cells become bulged and show long or short processes. The pocketings disappear and are replaced by openings which are covered by a fine fibrous network surrounding the vascular wall. The plexus-like arrangement of the subepithelial lymphatics of the tongue can well be demonstrated in corrosion casts. The vascular system consists for the most part of capillary vessels anastomosing like a net or terminating blindly in rootlets. The imprints of endothelial nuclei and cellular borders as well as of valve patterns in the casts enable a good differentiation between vascular and interstitial structures. The findings on the special morphological characteristics of the initial lymphatics are also discussed under functional aspects.
通过扫描电子显微镜对间隙固定组织和铸型标本中的大鼠舌上皮下淋巴管进行了检查。在低间隙压力下固定的组织标本中，内皮由宽而扁平的细胞组成，内皮边界呈锯齿状。在细胞间边界系统中，存在简单连接和重叠区域。后者呈现出袋状外观。在高达75mmHg的高间隙压力下固定的组织中，许多内皮细胞膨出并呈现出长或短的突起。袋状结构消失，取而代之的是被围绕血管壁的精细纤维网络覆盖的开口。舌上皮下淋巴管的丛状排列在铸型标本中能得到很好的显示。血管系统大部分由像网一样相互吻合或在小支中盲端终止的毛细血管组成。铸型标本中内皮细胞核、细胞边界以及瓣膜模式的印记使得血管结构和间质结构能够很好地区分。还从功能方面讨论了初始淋巴管特殊形态特征的研究结果。
