大鼠肾缺血期间核磁共振不可见的ADP的形成。
Formation of n.m.r.-invisible ADP during renal ischaemia in rats.
作者信息
Stubbs M, Freeman D, Ross B D
出版信息
Biochem J. 1984 Nov 15;224(1):241-6. doi: 10.1042/bj2240241.
Abstract
Measurement of the adenine nucleotide and inorganic phosphate content of normoxic and ischaemic kidney in vivo has been made, comparing enzymic assay (after freeze-clamping and acid extraction) with quantification by 31P-n.m.r. Both methods give similar results for ATP, and n.m.r. quantification of Pi gives a value 25-50% of that obtained by enzymic assay. ADP, which is largely invisible to n.m.r. in the normoxic kidney, remains invisible during ischaemia despite a 2-3 fold rise in enzymically assayed ADP. N.m.r. and enzymic assay of the acid extracts give similar values for all metabolites measured. The question of ADP binding in the kidney is discussed, as are the implications for the metabolic regulation of ADP-dependent reactions.
摘要
已对正常氧合和缺血肾脏中腺嘌呤核苷酸和无机磷酸盐的含量进行了体内测量，将酶法测定（冷冻钳夹和酸提取后）与通过³¹P-核磁共振定量进行比较。两种方法对ATP得出的结果相似，通过核磁共振对无机磷酸盐的定量得出的值是酶法测定值的25%-50%。在正常氧合肾脏中基本无法通过核磁共振检测到的ADP，在缺血期间尽管酶法测定的ADP升高了2-3倍，但仍无法检测到。对酸提取物进行的核磁共振和酶法测定对所有测量的代谢物得出相似的值。文中讨论了肾脏中ADP结合的问题以及对ADP依赖性反应代谢调节的影响。
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