大鼠肾脏活体磷核磁共振研究。

Phosphorus nuclear magnetic resonance study of the rat kidney in vivo.

作者信息

Balaban R S, Gadian D G, Radda G K

出版信息

Kidney Int. 1981 Nov;20(5):575-9. doi: 10.1038/ki.1981.179.

DOI:10.1038/ki.1981.179

Abstract

We show that phosphorus nuclear magnetic resonance (31P-NMR) can be used to study the metabolic state of kidneys in live, anesthetized rats without the need for surgery. To localize signals from the kidney, we used a radiofrequency surface coil in conjunction with the magnetic field profiling technique that is used for "topical magnetic resonance," (TMR). Signals are observed from phosphorus-containing metabolites including ATP and inorganic phosphate, and under certain conditions intracellular pH can be estimated. We suggest that the ratio of free ATP to free ADP is higher than the estimates of 1.5 to 2.0 obtained from freeze-clamping studies. Because of the important roles that ATP, ADP, inorganic phosphate, and pH are believed to play in cellular metabolism, it seems that 31P-NMR could be a powerful clinical tool.

摘要

我们表明，磷核磁共振（31P-NMR）可用于研究活体麻醉大鼠肾脏的代谢状态，而无需进行手术。为了定位来自肾脏的信号，我们使用了射频表面线圈，并结合了用于“局部磁共振”（TMR）的磁场分析技术。从含磷代谢物（包括ATP和无机磷酸盐）中观察到信号，并且在某些条件下可以估计细胞内pH值。我们认为，游离ATP与游离ADP的比率高于通过冷冻钳夹研究获得的1.5至2.0的估计值。由于ATP、ADP、无机磷酸盐和pH值被认为在细胞代谢中发挥重要作用，因此31P-NMR似乎可能是一种强大的临床工具。