Vunder P A, Murashev A N
Biull Eksp Biol Med. 1984 Nov;98(11):608-9.
Immersion of rat scrotum into water (41 degrees C) for 30 min leads to gross impairment of spermatogenesis. During incubation in Ringer-Krebs solution at 33 degrees C for 1h, fragments of the decapsulated testes taken from the heated rats secrete to the medium a high amount of substances that increase the optical density of the incubation liquid at 270 nm. This phenomenon can be recorded shortly after scrotum heating is completed. The optical density increases further 12 and 24 h after heating. During incubation, fragments of the testes heated in vivo also secrete a high amount of the lysosomal enzymes, acid phosphatase and cathepsin D. It is assumed that post-thermal degeneration of the spermatogenic epithelium may be determined by a change in the permeability of cellular and lysosomal membranes.
将大鼠阴囊浸入41摄氏度的水中30分钟会导致精子发生严重受损。在33摄氏度的林格 - 克雷布斯溶液中孵育1小时期间，取自受热大鼠的去包膜睾丸碎片会向培养基中分泌大量物质，这些物质会增加孵育液在270纳米处的光密度。这种现象在阴囊加热完成后不久即可记录到。加热后12小时和24小时光密度进一步增加。在孵育期间，体内受热的睾丸碎片也会分泌大量溶酶体酶、酸性磷酸酶和组织蛋白酶D。据推测，生精上皮的热后变性可能由细胞膜和溶酶体膜通透性的变化所决定。
