Fujimoto S, Hisada S
Jpn J Pharmacol. 1978 Feb;28(1):49-56. doi: 10.1254/jjp.28.49.
Effects of centrally affecting drugs on the diuretic and antidiuretic actions of intracerebroventricularly (i.c.v.) injected prostaglandin (PG) E2 in ethanol-anaesthetized rats were studied. PGE2, when injected i.c.v. at a dose of 1 nmole, produced diuresis followed by antidiuresis. When morphine (0.1 mM) was perfused i.c.v., urine outflow decreased and neither diuretic nor antidiuretic effects of i.c.v. PGE2 was apparent. The perfusions with picrotoxin, gamma-aminobutyric acid and L-glutamate inhibited either the diuretic or the antidiuretic effect of PGE2. On the other hand, when pentobarbital, diazepam, isoniazid and strychnine were perfused i.c.v., the diuretic action of PGE2 was diminished and antidiuresis in response to PGE2 remained unchanged. These results suggested that the diuretic and antidiuretic effects of PGE2 could be separated. The developed of the diuretic effect of PGE2 was completely blocked by amitriptyline and antidiuresis was increased. In rats pretreated i.c.v. with reserpine, the diuretic effect of PGE2 was prolonged and antidiuresis in response to PGE2 was not observed. An antidiuretic action of i.c.v. norepinephrine was not varied by reserpine. Mechanisms for both effects of PGE2 are discussed.
研究了中枢作用药物对乙醇麻醉大鼠脑室内（i.c.v.）注射前列腺素（PG）E2的利尿和抗利尿作用的影响。当以1纳摩尔的剂量脑室内注射PGE2时，会先产生利尿作用，随后出现抗利尿作用。当脑室内灌注吗啡（0.1 mM）时，尿流量减少，脑室内注射PGE2的利尿和抗利尿作用均不明显。用印防己毒素、γ-氨基丁酸和L-谷氨酸灌注可抑制PGE2的利尿或抗利尿作用。另一方面，当脑室内灌注戊巴比妥、地西泮、异烟肼和士的宁时，PGE2的利尿作用减弱，而对PGE2的抗利尿反应保持不变。这些结果表明PGE2的利尿和抗利尿作用可以分开。阿米替林完全阻断了PGE2的利尿作用，且抗利尿作用增强。在脑室内预先用利血平处理的大鼠中，PGE2的利尿作用延长，且未观察到对PGE2的抗利尿反应。脑室内注射去甲肾上腺素的抗利尿作用不受利血平影响。讨论了PGE2两种作用的机制。
