Koch T R, Cave D R, Ford H, Kirsner J
J Auton Nerv Syst. 1984 Dec;11(4):383-91. doi: 10.1016/0165-1838(84)90086-9.
Colonic tissues from patients with ulcerative colitis (UC), ileal tissue from patients with Crohn's disease (CD), and other ileocolonic disorders were radioenzymatically assayed for norepinephrine (NE) and dopamine (DA). These assays showed that the NE content of UC colon ranged from 3.2 to 4.3 ng/mg wet tissue, while in other colonic disorders it ranged from 3.0 to 5.3 ng/mg. Similarly, the DA content of UC colon ranged from 0.42 to 1.17 ng/mg wet tissue, while for other colonic disorders it ranged from 0.48 to 0.89 ng/mg. The same tissues were examined histochemically using glyoxylic acid condensation of catecholamines (CA) for the semi-quantitation of CA-containing structures. Examination of these structures revealed an apparent increase in their staining intensity in the myenteric plexus in ulcerative colitis as compared to other colonic disorders. Morphologically, the CA-containing structures of the colon appeared to be increased in UC, but determination of the NE and DA concentrations in colonic samples revealed no statistically significant differences in comparable regions of the colon.
对溃疡性结肠炎（UC）患者的结肠组织、克罗恩病（CD）患者的回肠组织以及其他回结肠疾病的组织进行了放射性酶法检测，以测定去甲肾上腺素（NE）和多巴胺（DA）的含量。这些检测表明，UC结肠的NE含量在湿组织中为3.2至4.3纳克/毫克，而在其他结肠疾病中则为3.0至5.3纳克/毫克。同样，UC结肠的DA含量在湿组织中为0.42至1.17纳克/毫克，而其他结肠疾病的DA含量为0.48至0.89纳克/毫克。使用儿茶酚胺（CA）的乙醛酸缩合反应对相同组织进行组织化学检查，以对含CA的结构进行半定量。对这些结构的检查显示，与其他结肠疾病相比，溃疡性结肠炎肌间神经丛中它们的染色强度明显增加。形态学上，结肠中含CA的结构在UC中似乎有所增加，但结肠样本中NE和DA浓度的测定显示，结肠相应区域在统计学上无显著差异。
