Suzuki A, Namba Y, Matsuura M, Horisawa A
J Hyg (Lond). 1984 Dec;93(3):567-73. doi: 10.1017/s0022172400065141.
Airborne contamination in an operating suite was studied with a slit sampler, settle plates and a light-scattering particle counter. In conventional operating rooms there was a significant difference between the empty rooms and rooms in use; the mean total bacterial count by a slit sampler changed from 1.1 in empty to 42.5 c.f.u./m3 in use (39 times increase), the settle plates count changed from 1.5 to 17.4 c.f.u./m2/min (12 times increase), and the mean total particle count changed from 56.9 to 546.7/l (10 times increase) respectively. The increase was caused mainly by persons present in the room. Another difference was found between zones in the operating suite; the bacterial count in the clean area doubled in the semi-clean area and further doubled in the dirty area in slit sampler count as well as settle plates count, and particle count in the clean area increased by 14 times in the semi-clean and dirty areas. This difference resulted from the different quality of the ventilating system. Air cleanliness of operating rooms in use by persons present in the room dropped to a level between the clean and the semi-clean area in spite of the high quality of the ventilating system. Bacterial species identified were mostly coagulase negative staphylococci and micrococci. Our study indicates that reduction of airborne contamination in an operating suite is accomplished by the combination of an efficient ventilating system and the restriction of the number of persons present in the room.
使用狭缝采样器、沉降平板和光散射粒子计数器对手术室中的空气传播污染进行了研究。在传统手术室中，空房间和使用中的房间之间存在显著差异；狭缝采样器测得的平均细菌总数从空房间时的1.1 c.f.u./m³ 增加到使用中的42.5 c.f.u./m³（增加了39倍），沉降平板计数从1.5 c.f.u./m²/分钟增加到17.4 c.f.u./m²/分钟（增加了12倍），平均总粒子数分别从56.9 /l 增加到546.7 /l（增加了10倍）。这种增加主要是由房间内的人员造成的。在手术室的不同区域之间还发现了另一个差异；在狭缝采样器计数和沉降平板计数中，清洁区域的细菌数量在半清洁区域翻倍，在污染区域进一步翻倍，清洁区域的粒子数在半清洁和污染区域增加了14倍。这种差异是由通风系统的不同质量导致的。尽管通风系统质量很高，但由于房间内人员的存在，使用中的手术室的空气清洁度下降到了清洁区域和半清洁区域之间的水平。鉴定出的细菌种类大多为凝固酶阴性葡萄球菌和微球菌。我们的研究表明，通过高效通风系统和限制房间内人员数量的结合，可以减少手术室中的空气传播污染。
