酯酶-16（es-16）：家鼠肾脏酯酶基因座的特性、多态性及与3号染色体的连锁关系

Esterase-16 (es-16): characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse.

作者信息

von Deimling O H, Schupp P, Otto J

出版信息

Biochem Genet. 1981 Dec;19(11-12):1091-9. doi: 10.1007/BF00484567.

Abstract

A polymorphism for an isozyme of a presumed arylesterase, esterase-16 (EC 3.1.1.2), has been detected in kidney, heart, and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinquished: the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10(-3) M p-chloromercuribenzoate, but not by 2. 10(-4) M bis-p-nitrophenyl phosphate or by 10(-3) M Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with alpha-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with three alleles, Es-16a, Es-16b, and ES-16c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3 Typing of 94 animals of the backcross (C57BL/10Sn x M. m. mol.) F1 x M. m. mol. revealed a recombination frequency of 8.51 /+- 2.9%.

摘要

通过等电聚焦和圆盘电泳法，在小家鼠（Mus musculus）的肾脏、心脏和脾脏中检测到一种假定的芳基酯酶同工酶酯酶-16（EC 3.1.1.2）的多态性。可区分出三种表型：ES-16A表型（等电点5.9）见于C57BL/10Sn及许多其他实验室近交系；ES-16B表型（等电点6.1）见于小家鼠的琉球亚种（M. m. molossinus）；ES-16C表型（等电点5.9；活性非常弱）见于秘鲁-科波克品系。酯酶-16受到10⁻³ M对氯汞苯甲酸的强烈抑制，但不受2×10⁻⁴ M双对硝基苯基磷酸酯或10⁻³ M醋甲唑胺的抑制。它用醋酸吲哚酚及其他产吲哚底物染色良好，但用醋酸α-萘酯染色较弱。酯酶-16完全不溶于水。它显然受一个结构基因座Es-16控制，分别有三个等位基因Es-16a、Es-16b和Es-16c。Es-16与3号染色体上的Car-1和Car-2紧密连锁。对回交（C57BL/10Sn×小家鼠琉球亚种）F1×小家鼠琉球亚种的94只动物进行分型，发现重组频率为8.51±2.9%。