Wolf R E
J Clin Invest. 1979 Apr;63(4):677-83. doi: 10.1172/JCI109350.
The proliferative response of human peripheral blood lymphocytes to phytohemagglutinin, concanavalin A, and pokeweed mitogen were suppressed by thymosin. Greatest decreases were observed when cells were preincubated with thymosin for 18 h before a 3-d culture with mitogen in the presence of thymosin. However, significant suppression also occurred when lymphocytes were preincubated for 2 h and cultured with thymosin or preincubated for either 2 or 18 h and washed free of thymosin before culture. These effects were related to the concentration of thymosin and time of exposure to thymosin but not merely to a delay in the response to mitogen or to toxicity. The suppression of mitogen-induced proliferation by thymosin appeared to result from effects of thymosin on a suppressor cell because lymphocytes incubated with thymosin did not acquire increased responsiveness to mitogens as did cells incubated for 18 h in its absence and because mixing thymosin-pretreated lymphocytes with cells not preincubated with thymosin resulted in decreased responsiveness to photohemagglutinin.
胸腺素可抑制人外周血淋巴细胞对植物血凝素、刀豆球蛋白A和商陆有丝分裂原的增殖反应。当细胞在有胸腺素存在的情况下与有丝分裂原进行3天培养前，先用胸腺素预孵育18小时，观察到最大程度的降低。然而，当淋巴细胞预孵育2小时并与胸腺素一起培养，或者预孵育2小时或18小时并在培养前洗去胸腺素时，也会出现显著的抑制作用。这些效应与胸腺素的浓度和暴露于胸腺素的时间有关，而不仅仅是对有丝分裂原反应的延迟或毒性。胸腺素对有丝分裂原诱导的增殖的抑制作用似乎是由于胸腺素对抑制细胞的作用，因为与胸腺素一起孵育的淋巴细胞并没有像在没有胸腺素的情况下孵育18小时的细胞那样获得对有丝分裂原的增强反应，并且因为将用胸腺素预处理的淋巴细胞与未用胸腺素预孵育的细胞混合会导致对植物血凝素的反应性降低。
