Tabata T, Iwabuchi M
Gene. 1984 Nov;31(1-3):285-9. doi: 10.1016/0378-1119(84)90223-3.
To determine whether there is structural variation among histone H4 genes in wheat, one (TH091) of the H4 genes that had been cloned from a wheat genomic DNA library was sequenced and compared with another H4 gene (TH011) which we had described previously [Tabata et al., Nucl. Acids Res. 11 (1983) 5865-5865]. Nucleotide sequence analysis revealed that there are 17 nucleotide replacements in the protein-coding region of two H4 genes, causing only one amino acid substitution: a glycine at position 4 (from the N terminus) in TH011 was replaced by an aspartic acid in TH091. S1 mapping, using total nuclear RNA from germinated seeds, indicated that the H4 gene was transcribed in vivo.
为了确定小麦组蛋白H4基因之间是否存在结构变异，对从小麦基因组DNA文库中克隆得到的一个H4基因（TH091）进行了测序，并与我们之前描述的另一个H4基因（TH011）[Tabata等人，《核酸研究》11（1983）5865 - 5865]进行比较。核苷酸序列分析表明，两个H4基因的蛋白质编码区有17个核苷酸替换，仅导致一个氨基酸替换：TH011中第4位（从N端起）的甘氨酸被TH091中的天冬氨酸取代。使用萌发种子的总核RNA进行S1作图表明，H4基因在体内被转录。
