弯曲杆菌属中超氧化物歧化酶的电泳分析

An electrophoretic analysis of superoxide dismutase in Campylobacter spp.

作者信息

Kikuchi H E, Suzuki T

出版信息

J Gen Microbiol. 1984 Nov;130(11):2791-6. doi: 10.1099/00221287-130-11-2791.

Abstract

Superoxide dismutase (SOD, superoxide:superoxide reductase, EC 1.15.1.1) activity was studied in 23 strains of Campylobacter spp. using disc polyacrylamide gel electrophoresis. Different enzyme patterns were observed with extracts of different species of Campylobacter; three migration bands were found in all strains of C. sputorum subsp. sputorum and C. sputorum subsp. bubulus (relative mobilities, Rm, 0.57, 0.76 and 0.85), and C. fetus subsp. fetus (Rm 0.60, 0.72 and 0.81), while four migration bands (Rm 0.52, 0.57, 0.73 and 0.82) were found in C. fetus subsp. venerealis. One band (Rm 0.73) was found in C. coli CIP 7080 and two bands (Rm 0.59, 0.73) in C. jejuni CIP 702. Superoxide dismutase activities were very high in the Campylobacter strains, especially in C. fetus subsp. fetus [specific activity 7.8-55.7 U (mg protein)-1] compared with those in Escherichia coli (1.5 U mg-1), Propionibacterium acnes (1.6 U mg-1) and Veillonella alcalescens (0.2 U mg-1).

摘要

利用圆盘聚丙烯酰胺凝胶电泳对23株弯曲杆菌属菌株的超氧化物歧化酶（SOD，超氧化物：超氧化物还原酶，EC 1.15.1.1）活性进行了研究。不同弯曲杆菌属菌种的提取物呈现出不同的酶谱；在所有唾液弯曲杆菌唾液亚种、唾液弯曲杆菌牛亚种和胎儿弯曲杆菌胎儿亚种菌株中均发现三条迁移带（相对迁移率，Rm，0.57、0.76和0.85），而在胎儿弯曲杆菌性病亚种中发现四条迁移带（Rm 0.52、0.57、0.73和0.82）。在大肠弯曲杆菌CIP 7080中发现一条带（Rm 0.73），在空肠弯曲杆菌CIP 702中发现两条带（Rm 0.59、0.73）。弯曲杆菌属菌株中的超氧化物歧化酶活性非常高，尤其是胎儿弯曲杆菌胎儿亚种[比活性7.8 - 55.7 U（mg蛋白）-1]，相比之下，大肠杆菌（1.5 U mg-1）、痤疮丙酸杆菌（1.6 U mg-1）和产碱韦荣球菌（0.2 U mg-1）中的活性则较低。