Goodman T G, Hoffman P S
J Clin Microbiol. 1983 Oct;18(4):825-9. doi: 10.1128/jcm.18.4.825-829.1983.
A rapid hydrogenase assay has been developed which may be useful in separating the species Campylobacter jejuni and C. coli from the subspecies C. fetus subsp. fetus and C. fetus subsp. venerealis. This assay employs the impermeant redox dye benzyl viologen, and positive determinations can be made within 20 min. All strains of C. jejuni and C. coli were found to be strongly hydrogenase positive. All strains of C. fetus subsp. fetus and C. fetus subsp. venerealis were negative for hydrogenase when the assay was performed at a benzyl viologen concentration of 2 mM and an incubation temperature of 30 degrees C. Some strains of C. fetus had low levels of hydrogenase as determined with cell extracts but were hydrogenase negative by the benzyl viologen assay. Since there are few rapid diagnostic tests available for screening Campylobacter isolates, we hope that the rapid hydrogenase assay will prove useful.
已开发出一种快速氢化酶检测方法，该方法可能有助于将空肠弯曲菌和大肠弯曲菌与胎儿弯曲菌胎儿亚种和胎儿弯曲菌性病亚种区分开来。该检测方法使用不透性氧化还原染料苄基紫精，20分钟内即可得出阳性结果。发现所有空肠弯曲菌和大肠弯曲菌菌株的氢化酶均呈强阳性。当在苄基紫精浓度为2 mM且孵育温度为30摄氏度的条件下进行检测时，所有胎儿弯曲菌胎儿亚种和胎儿弯曲菌性病亚种的菌株氢化酶均为阴性。一些胎儿弯曲菌菌株经细胞提取物检测显示氢化酶水平较低，但通过苄基紫精检测为氢化酶阴性。由于用于筛选弯曲菌分离株的快速诊断测试很少，我们希望这种快速氢化酶检测方法将被证明是有用的。
