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New rat cell line that is highly susceptible to transformation by several oncogenes.

作者信息

Liboi E, Caruso M, Basilico C

出版信息

Mol Cell Biol. 1984 Dec;4(12):2925-8. doi: 10.1128/mcb.4.12.2925-2928.1984.

DOI:10.1128/mcb.4.12.2925-2928.1984
PMID:6527690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369307/
Abstract

We describe here a new cell line, EL2, which spontaneously arose from primary rat embryo fibroblasts and has the distinctive property of being highly susceptible to a number of different transforming genes. The high susceptibility is expressed not only in high transformation frequencies but, most importantly, in an unusually high rate of growth of EL2 transformants under selective conditions, i.e., in soft agar or as foci. The biological characteristics of EL2 cells greatly accelerate the isolation of transformants from known oncogenes and could be useful to detect new transforming genes.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/164e/369307/adf88f9bc081/molcellb00154-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/164e/369307/adf88f9bc081/molcellb00154-0371-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/164e/369307/adf88f9bc081/molcellb00154-0371-a.jpg

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New rat cell line that is highly susceptible to transformation by several oncogenes.
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Focus formation in rat fibroblasts exposed to a tumour promoter after transfer of polyoma plt and myc oncogenes.在导入多瘤病毒plt和myc癌基因后，暴露于肿瘤启动子的大鼠成纤维细胞中的集落形成。
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Identification of the transformation-associated cell surface antigen expressed on the rat fetus-derived fibroblast.鉴定在大鼠胎儿来源的成纤维细胞上表达的转化相关细胞表面抗原。
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引用本文的文献

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本文引用的文献

1
SYRIAN HAMSTER FIBROBLAST CELL LINE BHK21 AND ITS DERIVATIVES.叙利亚仓鼠成纤维细胞系BHK21及其衍生物
Nature. 1964 Sep 26;203:1355-7. doi: 10.1038/2031355a0.
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Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.从T24膀胱癌细胞中分离出一种人类转化基因并进行初步鉴定。
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Common regulatory elements control gene expression from polyoma early and late promoters in cells transformed by chimeric plasmids.共同的调控元件控制着由嵌合质粒转化的细胞中多瘤病毒早期和晚期启动子的基因表达。
Mol Cell Biol. 1985 Aug;5(8):2070-9. doi: 10.1128/mcb.5.8.2070-2079.1985.
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Differential utilization of regulatory domains within the alpha 1(I) collagen promoter in osseous and fibroblastic cells.α1（I）型胶原蛋白启动子调控域在骨细胞和成纤维细胞中的差异利用
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Nature. 1982 Apr 1;296(5856):404-9. doi: 10.1038/296404a0.
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Proc Natl Acad Sci U S A. 1982 Jan;79(2):485-9. doi: 10.1073/pnas.79.2.485.
5
Transforming activity of human tumor DNAs.人类肿瘤DNA的转化活性。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181-4. doi: 10.1073/pnas.78.2.1181.
6
Cellular oncogenes and multistep carcinogenesis.细胞癌基因与多步骤致癌作用
Science. 1983 Nov 18;222(4625):771-8. doi: 10.1126/science.6356358.
7
Structural and immunological similarities between simian sarcoma virus gene product(s) and human platelet-derived growth factor.猿猴肉瘤病毒基因产物与人类血小板衍生生长因子之间的结构和免疫学相似性。
Nature. 1983;305(5935):605-8. doi: 10.1038/305605a0.
8
Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes.原代胚胎成纤维细胞的致瘤性转化至少需要两个协同作用的癌基因。
Nature. 1983;304(5927):596-602. doi: 10.1038/304596a0.
9
Cellular responsiveness to growth factors correlates with a cell's ability to express the transformed phenotype.
Cell. 1983 Jul;33(3):931-8. doi: 10.1016/0092-8674(83)90036-3.
10
Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein.仅表达中T蛋白的改变的多瘤病毒基因组对大鼠细胞的转化。
Nature. 1981 Aug 13;292(5824):595-600. doi: 10.1038/292595a0.