Axelson M, Sjövall J, Gustafsson B E, Setchell K D
J Endocrinol. 1984 Jul;102(1):49-56. doi: 10.1677/joe.0.1020049.
The dietary origin of the weak oestrogen equol (7-hydroxy-3-(4'-hydroxyphenyl)-chroman) present in human urine has been investigated using gas chromatography-mass spectrometry. Feeding experiments with different food constituents and monitoring the urinary excretion of equol revealed that soya food yields more than 0.1 mg urinary equol/g flour ingested. From this source the glucoside of daidzein (4',7-dihydroxyisoflavone) has been isolated and identified as a precursor of equol. Both equol and daidzein were characterized as monoglucuronide conjugates in human urine and the concentration of urinary equol exceeded the concentrations of the classical oestrogens by 100- to 1000-fold after ingestion of a single meal containing soya protein. The potential biological significance of this result is discussed.
利用气相色谱-质谱联用技术，对人体尿液中存在的弱雌激素雌马酚（7-羟基-3-(4'-羟基苯基)-苯并二氢吡喃）的饮食来源进行了研究。用不同食物成分进行喂养实验，并监测雌马酚的尿排泄情况，结果显示，食用大豆类食物后，每摄入1克面粉可产生超过0.1毫克的尿雌马酚。从该来源中分离并鉴定出大豆苷元（4',7-二羟基异黄酮）的葡糖苷为雌马酚的前体。在人体尿液中，雌马酚和大豆苷元均以单葡糖醛酸缀合物的形式存在，在摄入一顿含有大豆蛋白的餐后，尿雌马酚的浓度比经典雌激素的浓度高出100至1000倍。本文讨论了这一结果潜在的生物学意义。
