Kaji K, Seyama Y, Yamashita S
Nihon Yakurigaku Zasshi. 1984 Apr;83(4):325-9.
Active fractions that possess the hypocholesterolemic effect of iodine egg yolk lipid fraction have been investigated with male Wistar strain rats fed on a cholesterol-rich diet. Iodine egg yolk lipid was extracted by Folch's (chloroform: methanol) method and was refractionated into a neutral lipid (IEY-NL) fraction and a polar lipid (IEY-PL) fraction. The iodine content in each fraction was determined. The animals were kept on the cholesterol-rich diet for 5 days, and then they were fed the fractions in addition to the above diet for 10 more days. The hypocholesterolemic effect was seen most significantly with the IEY-NL fraction. The serum total cholesterol level in rats receiving this fraction was 70% as compared with that of the Ch group (fed on the cholesterol diet). However, the liver total cholesterol level was not affected or rather increased by the IEY-NL fraction. Another fraction (IEY-PL) showed no such effect. Iodine content in the IEY-NL fraction was quite low (0.2 ppm) compared with the other fractions. The daily dose in terms of iodine in the IEY-NL fraction was only the amount equivalent to 1/50 of the reported daily requirement for the rat. The IEY-NL fraction induced the hypocholesterolemic effect, though it contained only a trace of iodine.
利用喂食富含胆固醇饮食的雄性Wistar品系大鼠，对具有碘蛋黄脂质部分降胆固醇作用的活性部分进行了研究。碘蛋黄脂质通过Folch法（氯仿：甲醇）提取，并被重分为中性脂质（IEY-NL）部分和极性脂质（IEY-PL）部分。测定了各部分的碘含量。动物在富含胆固醇的饮食中饲养5天，然后除上述饮食外，再喂食这些部分10天。IEY-NL部分的降胆固醇作用最为显著。接受该部分的大鼠血清总胆固醇水平与Ch组（喂食胆固醇饮食）相比为70%。然而，肝脏总胆固醇水平未受IEY-NL部分影响，或反而有所升高。另一部分（IEY-PL）则无此作用。与其他部分相比，IEY-NL部分的碘含量相当低（0.2 ppm）。IEY-NL部分中碘的每日剂量仅相当于大鼠报告每日需求量的1/50。IEY-NL部分虽仅含微量碘，但仍诱导了降胆固醇作用。
