Merritt K, Brown S A, Sharkey N A
J Biomed Mater Res. 1984 Nov-Dec;18(9):1005-15. doi: 10.1002/jbm.820180905.
The binding of metal ions from salts and from corrosion products of 316 LVM stainless steel and MP-35 to blood cells and serum proteins was studied in vitro. In the first series of experiments, metal salts were added to whole blood and then the blood separated into red cells, white cells, and serum. Nickel from nickel chloride or corrosion products of stainless steel bound in very small quantities to blood cells. Cobalt from cobalt chloride bound to both red cells and white cells. Chromium from chromic chloride (Cr3+) bound to cells in very small quantities whereas chromium from potassium dichromate (Cr6+) and corrosion products showed very high to binding to red cells and some binding to white cells. In a second series of experiments the blood was separated into its components and then the metal salts were added and the binding pattern was identical. In a third series of experiments serum which had interacted with the metal salts or corrosion products was separated into its components by isoelectric focusing on polyacrylamide gels. Almost all of the metal, whatever the source, was detected in the albumin region of the gels indicating strong binding to albumin. These studies on the cell and protein binding of the metals help to explain the dissemination of corrosion products from the site of the implant and subsequent systemic responses by some individuals.
研究了316LVM不锈钢和MP - 35的盐类及腐蚀产物中的金属离子与血细胞和血清蛋白的体外结合情况。在第一系列实验中，将金属盐添加到全血中，然后将血液分离为红细胞、白细胞和血清。氯化镍中的镍或不锈钢腐蚀产物与血细胞的结合量极少。氯化钴中的钴与红细胞和白细胞都有结合。氯化铬（Cr3 +）中的铬与细胞的结合量极少，而重铬酸钾（Cr6 +）中的铬和腐蚀产物与红细胞的结合量非常高，与白细胞也有一些结合。在第二系列实验中，先将血液分离成其各个成分，然后添加金属盐，结合模式相同。在第三系列实验中，通过在聚丙烯酰胺凝胶上进行等电聚焦，将与金属盐或腐蚀产物相互作用后的血清分离成其各个成分。几乎所有金属，无论来源如何，都在凝胶的白蛋白区域被检测到，表明与白蛋白有强烈结合。这些关于金属与细胞和蛋白质结合的研究有助于解释植入部位腐蚀产物的扩散以及一些个体随后的全身反应。
