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Y1小鼠肾上腺肿瘤细胞中扩增的cKi-ras基因序列的结构与表达

Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells.

作者信息

George D L, Scott A F, Trusko S, Glick B, Ford E, Dorney D J

出版信息

EMBO J. 1985 May;4(5):1199-203. doi: 10.1002/j.1460-2075.1985.tb03760.x.

DOI:10.1002/j.1460-2075.1985.tb03760.x
PMID:4006913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC554324/
Abstract

A recombinant library of double minute chromosomal DNA, enriched in specific sequences that are amplified in Y1 mouse adrenal tumor cells, was used as a source of material to explore the structure and expression of amplified cKi-ras genes in these cells. From DNA sequence analysis of these cloned fragments, we found no evidence for the presence of point mutations previously demonstrated to be associated with activation of the transforming potential of ras genes. A comparison of the mouse gene with that of the homologous human cKi-ras2 gene reveals 94% nucleotide sequence homology within the coding regions and 97% homology for the predicted amino acid composition. Like the human gene, the mouse cKi-ras gene contains alternative 3' coding exons. Blot hybridization analyses of RNA revealed a preferential utilization of the more 3' of the two fourth coding exons in the generation of Y1 cKi-ras transcripts.

摘要

一个富含在Y1小鼠肾上腺肿瘤细胞中扩增的特定序列的双微体染色体DNA重组文库，被用作探索这些细胞中扩增的cKi-ras基因的结构和表达的材料来源。通过对这些克隆片段的DNA序列分析，我们没有发现先前证明与ras基因转化潜能激活相关的点突变存在的证据。将小鼠基因与同源的人类cKi-ras2基因进行比较，发现在编码区域内核苷酸序列同源性为94%，预测的氨基酸组成同源性为97%。与人类基因一样，小鼠cKi-ras基因包含选择性的3'编码外显子。RNA的印迹杂交分析显示，在Y1 cKi-ras转录本的产生中，两个第四编码外显子中更靠3'的外显子被优先利用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5fb/554324/0223d7a8ffa7/emboj00270-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5fb/554324/b7ef268ec907/emboj00270-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5fb/554324/0223d7a8ffa7/emboj00270-0122-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5fb/554324/b7ef268ec907/emboj00270-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a5fb/554324/0223d7a8ffa7/emboj00270-0122-a.jpg

相似文献

1
Structure and expression of amplified cKi-ras gene sequences in Y1 mouse adrenal tumor cells.Y1小鼠肾上腺肿瘤细胞中扩增的cKi-ras基因序列的结构与表达
EMBO J. 1985 May;4(5):1199-203. doi: 10.1002/j.1460-2075.1985.tb03760.x.
2
Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6.Y1小鼠肾上腺肿瘤细胞中的扩增DNA：与扩增的c-Ki-ras基因互补的cDNA的分离以及同源序列在小鼠6号染色体上的定位。
Nucleic Acids Res. 1984 Mar 26;12(6):2731-43. doi: 10.1093/nar/12.6.2731.
3
Amplified DNA sequences in Y1 mouse adrenal tumor cells: association with double minutes and localization to a homogeneously staining chromosomal region.Y1小鼠肾上腺肿瘤细胞中的扩增DNA序列：与双微体的关联及定位于均匀染色的染色体区域。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1597-601. doi: 10.1073/pnas.79.5.1597.
4
A cellular oncogene (c-Ki-ras) is amplified, overexpressed, and located within karyotypic abnormalities in mouse adrenocortical tumour cells.一种细胞癌基因（c-Ki-ras）在小鼠肾上腺皮质肿瘤细胞中发生扩增、过表达，并存在于核型异常中。
Nature. 1983;303(5917):497-501. doi: 10.1038/303497a0.
5
Cloning of DNA from double minutes of Y1 mouse adrenocortical tumor cells: evidence for gene amplification.从Y1小鼠肾上腺皮质肿瘤细胞的双微体中克隆DNA：基因扩增的证据
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6
Steroidogenic factor 1 (SF-1) and SP1 are required for regulation of bovine CYP11A gene expression in bovine luteal cells and adrenal Y1 cells.类固醇生成因子1（SF-1）和SP1是调节牛黄体细胞和肾上腺Y1细胞中牛CYP11A基因表达所必需的。
Mol Endocrinol. 1997 Feb;11(2):127-37. doi: 10.1210/mend.11.2.9890.
7
Transcriptional activation of cKi-ras proto-oncogene resulting from retroviral promoter insertion.逆转录病毒启动子插入导致cKi-ras原癌基因的转录激活。
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Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.小鼠c-Ki-ras基因启动子区域的结构与功能特征
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9
Amplified DNA with limited homology to myc cellular oncogene is shared by human neuroblastoma cell lines and a neuroblastoma tumour.人类神经母细胞瘤细胞系和一个神经母细胞瘤肿瘤中存在与myc细胞癌基因同源性有限的扩增DNA。
Nature. 1983;305(5931):245-8. doi: 10.1038/305245a0.
10
Characterization of a gene coamplified with Ki-ras in Y1 murine adrenal carcinoma cells that codes for a putative membrane protein.对Y1小鼠肾上腺癌细胞中与Ki-ras共扩增的一个基因的表征，该基因编码一种假定的膜蛋白。
Genomics. 1994 Mar 15;20(2):227-30. doi: 10.1006/geno.1994.1157.

引用本文的文献

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While K-ras is essential for mouse development, expression of the K-ras 4A splice variant is dispensable.虽然K-ras对小鼠发育至关重要，但K-ras 4A剪接变体的表达是可有可无的。
Mol Cell Biol. 2003 Dec;23(24):9245-50. doi: 10.1128/MCB.23.24.9245-9250.2003.
2
Mutations of K-ras oncogene in human adrenal tumours in Taiwan.台湾地区人类肾上腺肿瘤中K-ras癌基因的突变
Br J Cancer. 1998 Apr;77(7):1060-5. doi: 10.1038/bjc.1998.177.
3
Tumorigenic conversion of p53-deficient colon epithelial cells by an activated Ki-ras gene.激活的Ki-ras基因对p53缺陷型结肠上皮细胞的致瘤转化作用。

本文引用的文献

1
Cloning of DNA from double minutes of Y1 mouse adrenocortical tumor cells: evidence for gene amplification.从Y1小鼠肾上腺皮质肿瘤细胞的双微体中克隆DNA：基因扩增的证据
Cell. 1981 Apr;24(1):117-23. doi: 10.1016/0092-8674(81)90507-9.
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Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.T24膀胱癌转化基因的激活与单个氨基酸变化有关。
Nature. 1982 Dec 23;300(5894):762-5. doi: 10.1038/300762a0.
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Oncogenes in solid human tumours.人类实体瘤中的癌基因。
J Clin Invest. 1998 Apr 15;101(8):1572-80. doi: 10.1172/JCI919.
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High sequence similarity within ras exons 1 and 2 in different mammalian species and phylogenetic divergence of the ras gene family.不同哺乳动物物种中ras基因第1和第2外显子内的高度序列相似性以及ras基因家族的系统发育分歧。
Mamm Genome. 1998 Mar;9(3):214-9. doi: 10.1007/s003359900728.
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K-ras is an essential gene in the mouse with partial functional overlap with N-ras.K-ras是小鼠中的一个必需基因，与N-ras存在部分功能重叠。
Genes Dev. 1997 Oct 1;11(19):2468-81. doi: 10.1101/gad.11.19.2468.
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Structure of a Moloney murine leukemia virus-virus-like 30 recombinant: implications for transduction of the c-Ha-ras proto-oncogene.莫洛尼鼠白血病病毒-病毒样30重组体的结构：对c-Ha-ras原癌基因转导的影响
J Virol. 1993 Mar;67(3):1286-91. doi: 10.1128/JVI.67.3.1286-1291.1993.
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Pancreatic carcinomas of acinar and mixed acinar/ductal phenotypes in Ela-1-myc transgenic mice do not contain c-K-ras mutations.在Ela-1-myc转基因小鼠中，腺泡型及腺泡/导管混合型表型的胰腺癌不含有c-K-ras突变。
Am J Pathol. 1994 Sep;145(3):696-701.
8
Structural and functional characterization of the promoter region of the mouse c-Ki-ras gene.小鼠c-Ki-ras基因启动子区域的结构与功能特征
Mol Cell Biol. 1987 Jul;7(7):2592-6. doi: 10.1128/mcb.7.7.2592-2596.1987.
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Differential expression of the ras gene family in mice.ras基因家族在小鼠中的差异表达。
Mol Cell Biol. 1987 Apr;7(4):1535-40. doi: 10.1128/mcb.7.4.1535-1540.1987.
10
Enhanced c-Ki-ras expression associated with Friend virus integration in a bone marrow-derived mouse cell line.在一种源自骨髓的小鼠细胞系中，增强的c-Ki-ras表达与Friend病毒整合相关。
Proc Natl Acad Sci U S A. 1986 Mar;83(6):1651-5. doi: 10.1073/pnas.83.6.1651.
Nature. 1982 Dec 9;300(5892):539-42. doi: 10.1038/300539a0.
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A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.一个点突变导致了T24人膀胱癌癌基因获得转化特性。
Nature. 1982 Nov 11;300(5888):149-52. doi: 10.1038/300149a0.
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A catalogue of splice junction sequences.剪接连接序列目录。
Nucleic Acids Res. 1982 Jan 22;10(2):459-72. doi: 10.1093/nar/10.2.459.
6
Amplified DNA sequences in Y1 mouse adrenal tumor cells: association with double minutes and localization to a homogeneously staining chromosomal region.Y1小鼠肾上腺肿瘤细胞中的扩增DNA序列：与双微体的关联及定位于均匀染色的染色体区域。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1597-601. doi: 10.1073/pnas.79.5.1597.
7
Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene.c-bas/has人类原癌基因内的替代点突变导致转化特性的获得。
Nature. 1983 Jun 30;303(5920):775-9. doi: 10.1038/303775a0.
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Structure and activation of the human N-ras gene.人类N-ras基因的结构与激活
Cell. 1983 Sep;34(2):581-6. doi: 10.1016/0092-8674(83)90390-2.
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Three human transforming genes are related to the viral ras oncogenes.三种人类转化基因与病毒ras癌基因相关。
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2112-6. doi: 10.1073/pnas.80.8.2112.
10
Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-ras gene and localization of homologous sequences to mouse chromosome 6.Y1小鼠肾上腺肿瘤细胞中的扩增DNA：与扩增的c-Ki-ras基因互补的cDNA的分离以及同源序列在小鼠6号染色体上的定位。
Nucleic Acids Res. 1984 Mar 26;12(6):2731-43. doi: 10.1093/nar/12.6.2731.