位于12号染色体上的人类c-Ki-ras2原癌基因。
Human c-Ki-ras2 proto-oncogene on chromosome 12.
作者信息
Sakaguchi A Y, Naylor S L, Shows T B, Toole J J, McCoy M, Weinberg R A
出版信息
Science. 1983 Mar 4;219(4588):1081-3. doi: 10.1126/science.6823569.
A human colonic adenocarcinoma transforming gene, recently identified as a cellular homolog of the Kirsten sarcoma gene (v-ras), was used to assign the human cellular Kirsten ras2 gene to chromosome 12 by the Southern hybridization method. A single 640 base-pair Eco RI--Hind III fragment of the transforming gene, isolated by DNA transfection and molecular cloning, can detect a single Eco RI fragment (2.9 kilobase pairs) of DNA from phenotypically normal cells. The data suggest a constant chromosomal location of c-Ki-ras2.
一个最近被鉴定为柯斯顿肉瘤基因（v-ras）细胞同源物的人结肠腺癌转化基因，通过Southern杂交法被用于将人细胞柯斯顿ras2基因定位到12号染色体上。通过DNA转染和分子克隆分离得到的转化基因的一个单一的640碱基对的Eco RI-Hind III片段，能够检测到来自表型正常细胞的DNA的一个单一的Eco RI片段（2.9千碱基对）。这些数据表明c-Ki-ras2在染色体上的位置是恒定的。
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