Ehrlich K C, DeLucca A J, Ciegler A
Appl Environ Microbiol. 1984 Jul;48(1):1-4. doi: 10.1128/aem.48.1.1-4.1984.
Aspergillus niger was found to be the predominant fungal contaminant of stored cottonseed. Seven strains were isolated and grown on rice. The hexane-insoluble material from methylene chloride extracts of 2-week-old cultures contained components toxic to mice. Based on high-pressure thin-layer and liquid chromatographic analyses, the major components in the mixture were eight different naphtho-gamma-pyrones. Of these, the hydrated dimeric naphthopyrones aurasperones B and C occurred in higher yield than aurasperones A, iso-A, and D and the monomeric naphthopyrones flavasperone and rubrofusarin, all of which were present in the mixture. In addition, fonsecin monomethyl ether was isolated. This metabolite may be a precursor in the biosynthesis of the hydrated aurasperones; it has not been identified previously as a metabolite of A. niger. The relative amounts of the different naphthopyrones were dependent on both the growth substrate and the fungal isolate.
黑曲霉被发现是储存棉籽的主要真菌污染物。分离出七株菌株并在大米上培养。来自两周龄培养物二氯甲烷提取物的己烷不溶物含有对小鼠有毒的成分。基于高压薄层色谱和液相色谱分析，混合物中的主要成分是八种不同的萘并γ-吡喃酮。其中，水合二聚萘并吡喃酮金精三烯B和C的产量高于金精三烯A、异A和D以及单体萘并吡喃酮黄精三烯和红镰菌素，所有这些都存在于混合物中。此外，还分离出了丰塞菌素单甲醚。这种代谢物可能是水合金精三烯生物合成的前体；它以前未被鉴定为黑曲霉的代谢物。不同萘并吡喃酮的相对含量取决于生长底物和真菌分离株。
