通过改良的羟基苯并三唑磷酸三酯方法合成含硫代磷酸酯的DNA片段。
Synthesis of phosphorothioate-containing DNA fragments by a modified hydroxybenzotriazole phosphotriester approach.
作者信息
Marugg J E, van den Bergh C, Tromp M, van der Marel G A, van Zoest W J, van Boom J H
出版信息
Nucleic Acids Res. 1984 Dec 11;12(23):9095-110. doi: 10.1093/nar/12.23.9095.
Abstract
The phosphorothioylating agent which was obtained by treating 2,5-dichlorophenyl phosphorodichloridothioate with 1-hydroxy-6-nitrobenzotriazole proved to be very effective for the synthesis in solution and on a solid support of phosphorothioate-containing DNA fragments.
摘要
通过用1-羟基-6-硝基苯并三唑处理2,5-二氯苯基硫代磷酰二氯得到的硫代磷酸化试剂，在含硫代磷酸酯的DNA片段的溶液合成和固相合成中被证明非常有效。
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