镰刀菌属培养物中脱氧雪腐镰刀菌烯醇的分析。
Analysis of deoxynivalenol from cultures of Fusarium species.
作者信息
Pathre S V, Mirocha C J
出版信息
Appl Environ Microbiol. 1978 May;35(5):992-4. doi: 10.1128/aem.35.5.992-994.1978.
Abstract
Eight isolates of Fusarium roseum and three of Fusarium colmorum were found to produce deoxynivalenol in rice cultures. Deoxynivalenol was extracted with aqueous methanol (40%) and purified by partitioning with ethyl acetate and acetonitrile-petroleum ether (boiling point, 60--70 degrees C). The toxin was identified by gas chromatography/mass spectrometry and quantified by gas-liquid chromatography. High recoveries (80%) of deoxynivalenol were obtained from rice cultures, and as low as 0.250 microgram of the toxin per g was detected.
摘要
在水稻培养物中发现，8株粉红镰刀菌和3株禾谷镰刀菌能产生脱氧雪腐镰刀菌烯醇。用40%的甲醇水溶液提取脱氧雪腐镰刀菌烯醇，并通过用乙酸乙酯和乙腈 - 石油醚（沸点60 - 70℃）进行分配来纯化。通过气相色谱/质谱法鉴定该毒素，并通过气液色谱法定量。从水稻培养物中获得了较高的脱氧雪腐镰刀菌烯醇回收率（80%），并且检测到每克毒素低至0.250微克。
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